Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:74069
Morris-Wiman J, et al., Temporospatial distribution of matrix metalloproteinase and tissue inhibitors of matrix metalloproteinases during murine secondary palate morphogenesis. Anat Embryol (Berl). 2000 Aug;202(2):129-41
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6342623
|
|
Gene symbol:
|
Mmp9
|
|
Gene name:
|
matrix metallopeptidase 9
|
|
Antibody:
|
Anti-Mmp-9
|
|
Detection system:
|
fluorescein
|
|
Assay notes:
|
Fixation with acid alcohol.
|
|
|
Results
|
Specimen
2J:
embryonic day 12.0
(more  )
Specimen
2J:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: palatal shelf mesenchyme |
Present |
Regionally restricted |
2J
|
Expression localized to all regions of palatal mesenchyme, with increased expression within nasal and medial portions of central mesenchyme, and in lateral mesenchyme
|
| TS20: dental placode |
Present |
Regionally restricted |
2J
|
Enhanced expression within oral and presumptive tooth germ basement membrane.
|
| TS20: oral epithelium |
Present |
Regionally restricted |
2J
|
Enhanced expression within oral and presumptive tooth germ basement membrane.
|
Specimen
2K:
embryonic day 13.0
(more )
Specimen
2K:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf mesenchyme |
Strong |
Regionally restricted |
2K
|
Expression enhanced in nasal, adjacent dorsal medial, central and maxillary mesenchyme. Expression absent from mid-oral mesenchyme and lateral mesenchyme.
|
| TS21: palatal shelf epithelium |
Present |
Regionally restricted |
2K
|
Increased expression within nasal and oral basement membrane.
|
Specimen
2L:
embryonic day 14.0
(more )
Specimen
2L:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Strong |
Widespread |
2L
|
Increased expression within all regions of palatal mesenchyme, including ventral medial, mid-oral and lateral regions, and medial mesenchyme surrounding regressing palatal seam.
|
Specimen
gd 14-fused not shown:
embryonic day 14.0
(more )
Specimen
gd 14-fused not shown:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Weak |
Widespread |
|
Decreased expression within palatal mesenchyme, distributed throughout all regions of mesenchyme.
|
|
Analysis Tools