Antibody:	Anti-Mmp-2
Detection system:	fluorescein
Assay notes:	Fixation with acid alcohol.

Structure	Level	Pattern	Image	Note
TS20: palatal shelf mesenchyme	Present	Regionally restricted	2D	Expression within maxillary and adjacent regions of nasal palatal mesenchyme.
TS20: palatal shelf epithelium	Present	Regionally restricted	2D	Expression in basement membrane of oral epithelium and presumptive tooth germ.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Strong	Regionally restricted	2E	Significantly increased expression within all regions of palatal mesenchyme. Expression enhanced in nasal mesenchyme, extending into dorsal medial and central palatal mesenchyme. No expression in ventral medial, mid-oral and lateral mesenchyme.
TS21: tooth mesenchyme	Absent		2E	No expression in tooth germ mesenchyme
TS21: palatal shelf epithelium	Strong	Regionally restricted	2E	Expression in entire palatal epithelial basement membrane, slightly more intense in regions subjacent to nasal and lateral-most oral palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Strong	Regionally restricted	2F	Increased expression within dorsal medial mesenchyme adjacent to regressing palatal seam, extending in a band through central region of palate mesenchyme. No expression within ventral medial, mid-oral and lateral mesenchyme. Expressing invading mesenchyme of regressing palatal seam. Expression in nasal and maxillary region attributed to forming bone.
TS22: palatal shelf epithelium	Strong	Regionally restricted	2F	Strong expression in all regions of palatal epithelial basement membrane, with increased expression within remaining basement membrane.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Strong	Widespread		Increase in expression within all regions of mesenchyme. Strong expression in mesenchyme within palatal seam bridge. Expression also associated with osteogenic regions in central and maxillary emsencyme.