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Gene Expression Data
Assay Details
Assay
Reference: J:266902 Rafipay A, et al., Expression analysis of limb element markers during mouse embryonic development. Dev Dyn. 2018 Nov;247(11):1217-1226
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6342236
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: Anti-Neurofilament M (AB1987)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 5A: embryonic day 11.5 (more )
Note: After 4% PFA fixation, embryos were fixed in Dent's fixative.
Structure Level Pattern Image Note
TS19: forelimb nerve Present 5A
Specimen 5B: embryonic day 11.5 (more )
Note: After 4% PFA fixation, embryos were fixed in Dent's fixative.
Structure Level Pattern Image Note
TS20: forelimb nerve Absent 5B Hindlimb innervation at E11.5 was variable. Staining was not evident in this sample.
Specimen 5C: embryonic day 12.5 (more )
Note: After 4% PFA fixation, embryos were fixed in Dent's fixative.
Structure Level Pattern Image Note
TS20: forelimb nerve Present Regionally restricted 5C Main nerve trunks were present at E12.5, including branches that targeted to proximal tissues.
Specimen 5D: embryonic day 13.5 (more )
Note: After 4% PFA fixation, embryos were fixed in Dent's fixative.
Structure Level Pattern Image Note
TS21: forelimb nerve Present Regionally restricted 5D Distal nerve branches were seen, proximal patterning of nerves had become more intricate, and nerves had reached the handplate.
Specimen 5E: embryonic day 13.5 (more )
Note: After 4% PFA fixation, embryos were fixed in Dent's fixative.
Structure Level Pattern Image Note
TS21: hindlimb nerve Present Regionally restricted 5E Distal nerve branches were seen, proximal patterning of nerves had become more intricate, and nerves had reached the footplate.
Specimen 5F: embryonic day 14.5 (more )
Note: After 4% PFA fixation, embryos were fixed in Dent's fixative.
Structure Level Pattern Image Note
TS22: forelimb nerve Present Regionally restricted 5F' , 5F Nerves had reached digit tips, thicker nerve branches were visible, and patterning was substantially more complex.
Specimen 5G: embryonic day 14.5 (more )
Note: After 4% PFA fixation, embryos were fixed in Dent's fixative.
Structure Level Pattern Image Note
TS22: hindlimb nerve Present Regionally restricted 5G' , 5G Nerves had reached digit tips, thicker nerve branches were visible, and patterning was substantially more complex.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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