Probe:	Cdh5 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: hand	Present		4A
TS21: zeugopod	Present		4A
TS21: forelimb digit	Present	Regionally restricted	4A	Expression was detected along the digit rays, but no expression was observed in the distal digit tips.
TS21: forelimb interdigital region	Present	Regionally restricted	4A	Little expression was detected interdigitally, except stronger regions of expression at the digit base.
TS21: blood vessel	Present	Regionally restricted	4A	Blood vessels were visible in regions of developing cartilage.

Structure	Level	Pattern	Image	Note
TS21: hand	Present		4A'
TS21: zeugopod	Present		4A'
TS21: forelimb digit	Present	Regionally restricted	4A'	Expression was detected along the digit rays, but no expression was observed in the distal digit tips.
TS21: forelimb interdigital region	Present	Regionally restricted	4A'	Little expression was detected interdigitally, except stronger regions of expression at the digit base.
TS21: blood vessel	Present	Regionally restricted	4A'	Blood vessels were visible in regions of developing cartilage.

Structure	Level	Pattern	Image	Note
TS21: foot	Present		4B
TS21: zeugopod	Present		4B
TS21: hindlimb digit	Present	Regionally restricted	4B	Expression was detected along the digit rays, but no expression was observed in the distal digit tips.
TS21: hindlimb interdigital region	Present	Regionally restricted	4B	Little expression was detected interdigitally, except stronger regions of expression at the digit base.
TS21: blood vessel	Present	Regionally restricted	4B	Blood vessels were visible in regions of developing cartilage.

Structure	Level	Pattern	Image	Note
TS21: foot	Present		4B'
TS21: zeugopod	Present		4B'
TS21: hindlimb digit	Present	Regionally restricted	4B'	Expression was detected along the digit rays, but no expression was observed in the distal digit tips.
TS21: hindlimb interdigital region	Present	Regionally restricted	4B'	Little expression was detected interdigitally, except stronger regions of expression at the digit base.
TS21: blood vessel	Present	Regionally restricted	4B'	Blood vessels were visible in regions of developing cartilage.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	4C	Thick bands of expression radiated from the hand and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: hand	Present	Regionally restricted	4C	Thick bands of expression radiated from the hand and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: blood vessel	Present		4C

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	4C'	Thick bands of expression radiated from the hand and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: hand	Present	Regionally restricted	4C'	Thick bands of expression radiated from the hand and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: blood vessel	Present		4C'

Structure	Level	Pattern	Image	Note
TS22: foot	Present	Regionally restricted	4D	Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: hindlimb digit	Present	Regionally restricted	4D	Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: blood vessel	Present		4D

Structure	Level	Pattern	Image	Note
TS22: foot	Present	Regionally restricted	4D'	Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: hindlimb digit	Present	Regionally restricted	4D'	Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
TS22: blood vessel	Present		4D'

Structure	Level	Pattern	Image	Note
TS23: forelimb digit	Strong	Regionally restricted	4E , 4G	Strong bands of expression existed along the wrist, into the handplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: hand	Strong	Regionally restricted	4E , 4G	Strong bands of expression existed along the wrist, into the handplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: wrist	Strong		4E

Structure	Level	Pattern	Image	Note
TS23: forelimb digit	Strong	Regionally restricted	4E' , 4G'	Strong bands of expression existed along the wrist, into the handplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: hand	Strong	Regionally restricted	4E' , 4G'	Strong bands of expression existed along the wrist, into the handplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: wrist	Strong		4E'

Structure	Level	Pattern	Image	Note
TS23: foot	Strong	Regionally restricted	4F , 4H	Strong bands of expression existed along the ankle, into the footplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: hindlimb digit	Strong	Regionally restricted	4F , 4H	Strong bands of expression existed along the ankle, into the footplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: ankle	Strong		4F

Structure	Level	Pattern	Image	Note
TS23: foot	Strong	Regionally restricted	4H' , 4F'	Strong bands of expression existed along the ankle, into the footplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: hindlimb digit	Strong	Regionally restricted	4H' , 4F'	Strong bands of expression existed along the ankle, into the footplate, and along digit borders. No expression was observed in the distal digit tips.
TS23: ankle	Strong		4F'