Probe:	Myod1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: forelimb muscle	Strong	Regionally restricted	3A	Strong staining was detected in stylopod and zeugopod developing muscle blocks.
TS21: hand	Weak		3A

Structure	Level	Pattern	Image	Note
TS21: forelimb muscle	Strong	Regionally restricted	3A'	Strong staining was detected in stylopod and zeugopod developing muscle blocks.
TS21: hand	Weak		3A'

Structure	Level	Pattern	Image	Note
TS21: hindlimb muscle	Strong	Regionally restricted	3B	Strong staining was detected in stylopod and zeugopod developing muscle blocks.
TS21: foot	Weak		3B

Structure	Level	Pattern	Image	Note
TS21: hindlimb muscle	Strong	Regionally restricted	3B'	Strong staining was detected in stylopod and zeugopod developing muscle blocks.
TS21: foot	Weak		3B'

Structure	Level	Pattern	Image	Note
TS22: zeugopod	Strong	Regionally restricted	3C	Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
TS22: forelimb muscle	Strong	Regionally restricted	3C	Strong expression was detected in bands of developing forearm muscle.
TS22: hand	Present	Regionally restricted	3C	The handplate showed expression in muscle bands from the center radiating to digit bases.
TS22: wrist	Present	Regionally restricted	3C	A thin band of expression through the middle of the wrist connected expression in the zeugopod to the autopod in the forelimb.

Structure	Level	Pattern	Image	Note
TS22: zeugopod	Strong	Regionally restricted	3C'	Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
TS22: forelimb muscle	Strong	Regionally restricted	3C'	Strong expression was detected in bands of developing forearm muscle.
TS22: hand	Present	Regionally restricted	3C'	The handplate showed expression in muscle bands from the center radiating to digit bases.
TS22: wrist	Present	Regionally restricted	3C'	A thin band of expression through the middle of the wrist connected expression in the zeugopod to the autopod in the forelimb.

Structure	Level	Pattern	Image	Note
TS22: zeugopod	Strong	Regionally restricted	3D	Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
TS22: hindlimb muscle	Strong	Regionally restricted	3D	Strong expression was detected in bands of developing leg muscle.
TS22: foot	Present	Regionally restricted	3D	The footplate showed expression in muscle bands from the center radiating to digit bases.

Structure	Level	Pattern	Image	Note
TS22: zeugopod	Strong	Regionally restricted	3D'	Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
TS22: hindlimb muscle	Strong	Regionally restricted	3D'	Strong expression was detected in bands of developing leg muscle.
TS22: foot	Present	Regionally restricted	3D'	The footplate showed expression in muscle bands from the center radiating to digit bases.

Structure	Level	Pattern	Image	Note
TS23: autopod	Strong	Regionally restricted	3E , 3G	Expression at E15.5 was similar to that at E14.5.
TS23: zeugopod	Strong	Regionally restricted	3E , 3G	Expression at E15.5 was similar to that at E14.5.
TS23: wrist	Strong	Regionally restricted	3G , 3E	The band of expression connecting the zeugopod and autopod was stronger in the forelimb of E15.5 compared to E14.5.
TS23: forelimb muscle	Strong	Regionally restricted	3G , 3E	Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.

Structure	Level	Pattern	Image	Note
TS23: autopod	Strong	Regionally restricted	3E' , 3G'	Expression at E15.5 was similar to that at E14.5.
TS23: zeugopod	Strong	Regionally restricted	3E' , 3G'	Expression at E15.5 was similar to that at E14.5.
TS23: wrist	Strong	Regionally restricted	3E' , 3G'	The band of expression connecting the zeugopod and autopod was stronger in the forelimb of E15.5 compared to E14.5.
TS23: forelimb muscle	Strong	Regionally restricted	3E' , 3G'	Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.

Structure	Level	Pattern	Image	Note
TS23: autopod	Strong	Regionally restricted	3H , 3F	Expression at E15.5 was similar to that at E14.5.
TS23: zeugopod	Strong	Regionally restricted	3H , 3F	Expression at E15.5 was similar to that at E14.5.
TS23: hindlimb muscle	Strong	Regionally restricted	3H , 3F	Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.

Structure	Level	Pattern	Image	Note
TS23: autopod	Strong	Regionally restricted	3F' , 3H'	Expression at E15.5 was similar to that at E14.5.
TS23: zeugopod	Strong	Regionally restricted	3F' , 3H'	Expression at E15.5 was similar to that at E14.5.
TS23: hindlimb muscle	Strong	Regionally restricted	3H' , 3F'	Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.