Probe:	Scleraxis probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: hand	Present	Widespread	2A	Broad expression throughout the early autopod was noted.
TS20: forelimb digit	Weak		2A

Structure	Level	Pattern	Image	Note
TS20: hand	Present	Widespread	2A'	Broad expression throughout the early autopod was noted.
TS20: forelimb digit	Weak		2A'

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Widespread	2B	Broad expression throughout the early autopod was noted.
TS20: hindlimb digit	Weak		2B

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Widespread	2B'	Broad expression throughout the early autopod was noted.
TS20: hindlimb digit	Weak		2B'

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Non-Uniform	2C	Expression was detected in the handplate.
TS21: forelimb digit	Present		2C	Expression was detected in digit rays with thicker regions of expression at presumptive joint sites.
TS21: hand joint	Present		2C	Expression domains at presumptive joint sites of the forelimb were wider compared to the hindlimb.

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Non-Uniform	2C'	Expression was detected in the handplate.
TS21: forelimb digit	Present		2C'	Expression was detected in digit rays with thicker regions of expression at presumptive joint sites.
TS21: hand joint	Present		2C'	Expression domains at presumptive joint sites of the forelimb were wider compared to the hindlimb.

Structure	Level	Pattern	Image	Note
TS21: foot	Present	Non-Uniform	2D	Expression was detected in the footplate, and in digit rays with thicker regions of expression at presumptive joint sites.
TS21: foot joint	Present		2D
TS21: hindlimb digit	Present		2D

Structure	Level	Pattern	Image	Note
TS21: foot	Present	Non-Uniform	2D'	Expression was detected in the footplate, and in digit rays with thicker regions of expression at presumptive joint sites.
TS21: foot joint	Present		2D'
TS21: hindlimb digit	Present		2D'

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	2E	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
TS22: hand	Present	Regionally restricted	2E	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	2E'	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
TS22: hand	Present	Regionally restricted	2E'	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.

Structure	Level	Pattern	Image	Note
TS22: foot	Present	Regionally restricted	2F	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
TS22: hindlimb digit	Present	Regionally restricted	2F	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.

Structure	Level	Pattern	Image	Note
TS22: foot	Present	Regionally restricted	2F'	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
TS22: hindlimb digit	Present	Regionally restricted	2F'	Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.

Structure	Level	Pattern	Image	Note
TS22: connective tissue	Present	Regionally restricted	2G	Expression was detected in flexor tendons at the ventral side.

Structure	Level	Pattern	Image	Note
TS23: forelimb digit	Present	Non-Uniform	2K , 2H	Expression in distinct bands extended along the digits, with strong areas of expression persisting around developing joint regions.

Structure	Level	Pattern	Image	Note
TS23: forelimb digit	Present	Non-Uniform	2H' , 2K'	Expression in distinct bands extended along the digits, with strong areas of expression persisting around developing joint regions.

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit	Present	Non-Uniform	2L , 2I	Expression in distinct bands extended along the digits, with strong areas of expression persisting around developing joint regions.

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit	Present	Non-Uniform	2L' , 2I'	Expression in distinct bands extended along the digits, with strong areas of expression persisting around developing joint regions.

Structure	Level	Pattern	Image	Note
TS23: connective tissue	Present	Regionally restricted	2J	Expression was detected in flexor tendons at the ventral side.