Probe:	Gdf5 cDNA9
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: hand joint	Present	Regionally restricted	1A	Expression was detected along rays in presumptive joint regions that develop to separate rays into proximal, intermediate, and distal phalanges.

Structure	Level	Pattern	Image	Note
TS21: hand joint	Present	Regionally restricted	1A'	Expression was detected along rays in presumptive joint regions that develop to separate rays into proximal, intermediate, and distal phalanges.

Structure	Level	Pattern	Image	Note
TS21: foot joint	Present	Regionally restricted	1B	Expression was detected along rays in presumptive joint regions that develop to separate rays into proximal, intermediate, and distal phalanges.

Structure	Level	Pattern	Image	Note
TS21: foot joint	Present	Regionally restricted	1B'	Expression was detected along rays in presumptive joint regions that develop to separate rays into proximal, intermediate, and distal phalanges.

Structure	Level	Pattern	Image	Note
TS22: carpal-metacarpal joint	Present		1C	Expression was visible as thick bands in joint interzones.
TS22: forelimb distal interphalangeal joint	Present		1C	Expression was visible as thick bands in joint interzones.
TS22: metacarpal-phalangeal joint	Present		1C	Expression was visible as thick bands in joint interzones.

Structure	Level	Pattern	Image	Note
TS22: carpal-metacarpal joint	Present		1C'	Expression was visible as thick bands in joint interzones.
TS22: forelimb distal interphalangeal joint	Present		1C'	Expression was visible as thick bands in joint interzones.
TS22: metacarpal-phalangeal joint	Present		1C'	Expression was visible as thick bands in joint interzones.

Structure	Level	Pattern	Image	Note
TS22: hindlimb distal interphalangeal joint	Present		1D	Expression was visible as thick bands in joint interzones.
TS22: metatarsal-phalangeal joint	Present		1D	Expression was visible as thick bands in joint interzones.
TS22: tarsal-metatarsal joint	Present		1D	Expression was visible as thick bands in joint interzones.

Structure	Level	Pattern	Image	Note
TS22: hindlimb distal interphalangeal joint	Present		1D'	Expression was visible as thick bands in joint interzones.
TS22: metatarsal-phalangeal joint	Present		1D'	Expression was visible as thick bands in joint interzones.
TS22: tarsal-metatarsal joint	Present		1D'	Expression was visible as thick bands in joint interzones.

Structure	Level	Pattern	Image	Note
TS22: forelimb distal interphalangeal joint	Strong	Regionally restricted	1E	Expression was detected in the joint interzones in distal interphalangeal joints.

Structure	Level	Pattern	Image	Note
TS23: carpal-metacarpal joint	Present		1I , 1F	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: forelimb distal interphalangeal joint	Present		1I , 1F	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: forelimb proximal interphalangeal joint	Present		1I , 1F	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: metacarpal-phalangeal joint	Present		1I , 1F	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.

Structure	Level	Pattern	Image	Note
TS23: carpal-metacarpal joint	Present		1I' , 1F'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: forelimb distal interphalangeal joint	Present		1I' , 1F'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: forelimb proximal interphalangeal joint	Present		1I' , 1F'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: metacarpal-phalangeal joint	Present		1I' , 1F'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.

Structure	Level	Pattern	Image	Note
TS23: hindlimb distal interphalangeal joint	Present		1G , 1J	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: hindlimb proximal interphalangeal joint	Present		1G , 1J	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: metatarsal-phalangeal joint	Present		1G , 1J	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: tarsal-metatarsal joint	Present		1G , 1J	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.

Structure	Level	Pattern	Image	Note
TS23: hindlimb distal interphalangeal joint	Present		1G' , 1J'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: hindlimb proximal interphalangeal joint	Present		1G' , 1J'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: metatarsal-phalangeal joint	Present		1G' , 1J'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.
TS23: tarsal-metatarsal joint	Present		1G' , 1J'	The joint patterning at E15.5 became more apparent as thinner bands in more defined joints.

Structure	Level	Pattern	Image	Note
TS23: hindlimb distal interphalangeal joint	Strong	Regionally restricted	1H	Strong expression was detected in joint interzones in the distal interphalangeal joints.