Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Acta2-GCaMP8.1/mVermilion)B34-4Mik
Name:	transgene insertion B34-4, Michael I Kotlikoff
MGI ID:	MGI:6324941
Synonyms:	B34-Acta2BAC-GCaMP8.1_mVermilion
Transgene:	Tg(Acta2-GCaMP8.1/mVermilion)B34-4Mik  Location: unknown
Alliance:	Tg(Acta2-GCaMP8.1/mVermilion)B34-4Mik page

Strain of Origin:

C57BL/6J x SJL/J

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: Acta2-GCaMP8.1-mVermilion transgenic mice (also called Acta2-GCaMP8.1/mVermilion or Acta2-GCaMP8.1-mVermilion) were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus). The ~175 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-370F21 was obtained; containing the entire actin alpha 2 locus (Acta2) as well as ~92 kbp of 5' flanking sequences (including the complete Fas locus) and ~68 kbp of 3' flanking sequences (including the complete Stambpl1 locus). Using homologous recombination/BAC recombineering, a 2993 bp GCaMP8.1/mVermilion-SV40pA construct (see details below) was inserted into the ATG start site of the BAC Acta2 gene (replacing the initiation codon of Acta2 in exon 2). No other loci on the BAC were altered. The 11,612 bp BAC vector (pBACe3.6) contained a chloramphenicol selection cassette. (J:314125)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
All:	1 reference(s)