Tg(Cdh5-RHO/Adrb2,-lacZ)B27-3Mik
Transgene Detail
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| Symbol: |
Tg(Cdh5-RHO/Adrb2,-lacZ)B27-3Mik |
| Name: |
transgene insertion B27-3, Michael I Kotlikoff |
| MGI ID: |
MGI:6324935 |
| Synonyms: |
B27-Cdh5BAC-Optobeta 2AR_IRES_lacZ |
| Transgene: |
Tg(Cdh5-RHO/Adrb2,-lacZ)B27-3Mik Location: unknown
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| Alliance: |
Tg(Cdh5-RHO/Adrb2,-lacZ)B27-3Mik page
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| Strain of Origin: |
FVB/N X B6(Cg)-Tyrc-2J/J
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| Transgene Type: |
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Transgenic (Inserted expressed sequence, Reporter) |
| Mutation: |
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Insertion
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Mutation details: Cdh5-Optoβ2AR-IRES-lacZ transgenic mice (also called Cdh5-Optoβ2AR-IRES-lacZ or Cdh5-Opto-β2AR-IRES-lacZ) were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus). The ~200 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-453P1 was obtained; containing the entire cadherin 5 locus (Cdh5) as well as ~124 kbp of 5' flanking sequences (including the complete Gm32728, Gm39236 and Gm8748 predicted gene loci) and ~33 kbp of 3' flanking sequences (including a 5' portion of the Bean1 locus). Using homologous recombination/BAC recombineering, a 5990 bp Optoβ2AR-IRES-lacZ-pA construct (Optoβ2AR, internal ribosome entry site, β-galactosidase and an SV40 polyadenylation signal) was inserted into the ATG start site of the BAC Cdh5 gene (replacing the initiation codon of Cdh5 in exon 2).
(J:314125)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858 |
| All: |
1 reference(s) |
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Analysis Tools
