Gene Expression Data
Assay Details
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Assay
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Reference:
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J:86841
Elms P, et al., Zic2 is required for neural crest formation and hindbrain patterning during mouse development. Dev Biol. 2003 Dec 15;264(2):391-406
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6324692
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Gene symbol:
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Msx1
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Gene name:
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msh homeobox 1
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Probe:
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Probe 1
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Probe preparation:
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Antisense RNA
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Assay notes:
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Two probes were described, but the one used in this assay was not detailed. Probe2 MGI:1275945 may have been used in this assay.
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Results
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Specimen
6A:
embryonic day 9.0
(more  )
Note: 14-somite stage
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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14-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural tube |
Present |
Regionally restricted |
6A
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Expression in dorsal part of closed neural tube.
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Specimen
6B:
embryonic day 9.0; Zic2Ku/ Zic2Ku
(more )
Note: 15-somite stage
Specimen
6B:
(close )
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Genetic Background:
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C3.CAnNCrl-Zic2Ku
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Mutant Allele(s):
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Zic2Ku/Zic2Ku
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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15-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural tube |
Present |
Regionally restricted |
6B
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Expression remains restricted to dorsal neural tube. No difference in size of expressing region compared with wild type.
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Specimen
6G:
embryonic day 9.0
(more )
Note: 14-somite stage
Specimen
6G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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14-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural plate |
Present |
Regionally restricted |
6G
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Expression at margin of neural plate.
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Specimen
6H:
embryonic day 9.0; Zic2Ku/ Zic2Ku
(more )
Note: 15-somite stage
Specimen
6H:
(close )
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Genetic Background:
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C3.CAnNCrl-Zic2Ku
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Mutant Allele(s):
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Zic2Ku/Zic2Ku
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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15-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural plate |
Absent |
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6H
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Expression lost in regions where neural tube has yet to close.
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Analysis Tools
