Probe:	NF-L probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3G/H	Expressed in newly differentiated motor neurons.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3O/P	Expressed in newly differentiated motor neurons.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Non-Uniform	5D	Expressed in discrete areas, in regions containing differentiated neurons.
TS23: medulla oblongata	Present		5D	Presumably in large motor neurons.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Strong		6G/H

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		9D
TS27: hippocampus	Present		9D

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex marginal zone	Present		10G/H
TS27: hippocampus CA1	Strong		10G/H
TS27: hippocampus CA2	Strong		10G/H
TS27: hippocampus CA3	Strong		10G/H

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Regionally restricted	11G/H	Expressed in a region inside the external germinal layer, presumably the internal germinal layer and/or the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: amygdaloid nucleus	Present		12D
TS28: deep cerebellar nucleus	Present		12D
TS28: hippocampus	Present		12D
TS28: hippocampus pyramidal cell layer	Present		12D
TS28: thalamus	Present		12D

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Absent

Structure	Level	Pattern	Image	Note
TS27: cerebellum molecular layer	Present
TS27: cranial ganglion	Present
TS27: deep cerebellar nucleus	Present
TS27: dorsal motor nucleus of vagus nerve	Present
TS27: hippocampus pyramidal cell layer	Present
TS27: thalamic nucleus	Present
TS27: trigeminal V motor nucleus	Present

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted		Expressed in the same areas as before, but their expression also extended more rostrally and covered a larger area of the cortical plate, including large neurons in the pyramidal layer.
TS28: entorhinal cortex	Strong	Regionally restricted		Expressed in the laminae entorhinalis and stratum pyramidale subiculi.
TS28: hippocampus	Strong

Structure	Level	Pattern	Image	Note
TS28: cranial ganglion	Present
TS28: dorsal motor nucleus of vagus nerve	Present
TS28: trigeminal V motor nucleus	Present