Probe:	Rat.401.gt10.16
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	1A	Expressed symmetrically at both sides of the midline.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		1B

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Non-Uniform	1C	Expressed more intensely at the pial side.
TS15: branchial arch	Present		1C

Structure	Level	Pattern	Image	Note
TS12: neural plate	Present	Non-Uniform	1D	Expressed largely in the columnar neuroepithelial cells. The signal was stronger in the parts of the cells facing the mesoderm.

Structure	Level	Pattern	Image	Note
TS13: neural groove	Present	Non-Uniform	1E	Higher expression at the pial side.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Non-Uniform	1F	Expressed more intensely at the pial side.
TS15: migrating neural crest	Present		1F	Expressed on both sides of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3A/B	The expression level was highest at the pial side and was localized to the outer-most region.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3I/J	The expression level was highest at the pial side. The majority of staining was observed outside motor neurons and the fiber tracts in the mantle layer, but was clearly inside the mesenchymal cells surrounding the neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	4A/B	Expressed in a region outside the mantle layer. Considarably lower levels of expression were detected on the luminal side.

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present	Regionally restricted	5A	Expressed mostly in the areas surrounding the lateral vesicle. No expression in more caudal regions.
TS23: cerebellum	Present		5A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		5E/F
TS23: telencephalon subventricular zone	Present		5E/F
TS23: telencephalon ventricular layer	Present		5E/F
TS23: cerebral cortex	Strong	Regionally restricted	5E/F	Expressed in areas outside the marginal zone, close to the bones of the skull, which contains the pial endfeet of radial glial cells spanning the developing telencephalon.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Weak	Uniform	6A/B

Structure	Level	Pattern	Image	Note
TS23: olfactory system	Absent		7A/B

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		9A	Reduced expression.
TS27: telencephalon	Present		9A	Reduced expression. Expressed in a region near the lateral ventricles.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex subventricular zone	Weak	Regionally restricted	10A/B	Expressed in the areas surrounding the lateral vesicle.
TS27: cerebral cortex ventricular zone	Weak	Regionally restricted	10A/B	Expressed in the areas surrounding the lateral vesicle.
TS27: brain meninges	Present	Regionally restricted	10A/B	Expressed in a region between hippocampus and cortex.
TS27: hippocampus	Trace		10A/B	Very low expression.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Uniform	11A/B	Relatively uniformly distributed over the different regions including the white matter, but lower over the external germinal layer.
TS27: cerebellum white matter	Present		11A/B

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Absent		12A
TS28: brain meninges	Present	Regionally restricted	12A	Expressed in the membranes between cerebellum and cortex and near hippocampus, the recess of the third ventricle.

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Present		12E/F

Structure	Level	Pattern	Image	Note
TS17: myotome	Present
TS17: dorsal root ganglion	Weak
TS17: migrating neural crest	Weak

Structure	Level	Pattern	Image	Note
TS23: tongue	Strong
TS23: choroid plexus	Absent

Structure	Level	Pattern	Image	Note
TS27: brain meninges	Present	Regionally restricted		Expressed in the meninges surrounding the brain, but not in the hippocampus.
TS27: choroid plexus	Present

Structure	Level	Pattern	Image	Note
TS28: brain meninges	Present			Expressed in the same areas as at P0, but at generally lower levels.
TS28: cerebellum	Present			Expressed in the same areas as at P0, but at generally lower levels.
TS28: hippocampus	Absent