Probe:	SPC4 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: follicle cell of secondary follicle	Strong		3A/B	Expressed in granulosa cells.

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Strong		3D/E

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	3D/E	Expressed in maternal decidual cells surrounding the implantation site.

Structure	Level	Pattern	Image	Note
TS11: chorion	Present		3F

Structure	Level	Pattern	Image	Note
TS13: gut	Present		3G , 3H/I	Highest expression.
TS13: surface ectoderm	Present	Regionally restricted	3G , 3H/I	Highest level of expression was observed laterally to the primitive gut.

Structure	Level	Pattern	Image	Note
TS15: heart	Strong		4A/B	Expressed in all the tissues.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Strong		4C
TS19: neural tube floor plate	Strong		4C

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Strong		4D/E

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present		6C
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	6C	Expressed in the mesenchyme underlying the apical ectodermal ridge, although at significantly lower levels.

Structure	Level	Pattern	Image	Note
TS20: humerus pre-cartilage condensation	Present		7A/B
TS20: radius-ulna pre-cartilage condensation	Present		7A/B
TS20: forelimb digit mesenchyme	Present	Regionally restricted	7C/D , 7A/B	Expressed in mesenchymal cells at the distal tip of the limb.
TS20: forelimb digit epithelium	Absent		7C/D , 7A/B

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region mesenchyme	Present		7H , 7I
TS20: forelimb digit mesenchyme	Present	Regionally restricted	7I , 7H	Expressed in the cartilage primordia of the future digits.

Structure	Level	Pattern	Image	Note
TS24: hindlimb digit	Strong	Regionally restricted	8C/D , 8A/B	Expressed in hypertrophic chondrocytes within the primary ossification centers. almost undetectable in resting and proliferating chondrocytes at the periphery of the cartilage.
TS24: epidermis	Present		8A/B

Structure	Level	Pattern	Image	Note
TS24: intestinal villus	Present	Regionally restricted	8E/F	Expressed in the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS17: heart	Strong	Widespread		Expressed in all the tissues.

Structure	Level	Pattern	Image	Note
TS24: limb digit	Strong	Regionally restricted		Expressed in hypertrophic chondrocytes within the primary ossification centers. almost undetectable in resting and proliferating chondrocytes at the periphery of the cartilage.
TS24: limb long bone	Strong	Regionally restricted		Expressed in hypertrophic chondrocytes within the primary ossification centers. almost undetectable in resting and proliferating chondrocytes at the periphery of the cartilage.