Gene Expression Data
Assay Details
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Assay
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Reference:
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J:70735
Lee CS, et al., Transdifferentiation of the ventral retinal pigmented epithelium to neural retina in the growth arrest specific gene 1 mutant. Dev Biol. 2001 Aug 1;236(1):17-29
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6323046
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Gene symbol:
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Dct
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Gene name:
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dopachrome tautomerase
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Probe:
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Tyrp2 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5B:
embryonic day 9.5
(more  )
Note: The genetic background of the specimen was either wild type or heterozygous.
Specimen
5B:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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The genetic background of the specimen was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic vesicle |
Present |
Regionally restricted |
5B
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Expression in dorsal domains in optic vesicles.
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Specimen
5B':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gas1tm1Fan/Gas1tm1Fan
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic vesicle |
Present |
Regionally restricted |
5B'
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Expression in dorsal domain in optic vesicle. No discernable difference between control and mutant eyes.
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Specimen
5D:
embryonic day 10.5
(more )
Note: The genetic background of the specimen was either wild type or heterozygous.
Specimen
5D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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The genetic background of the specimen was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup outer layer |
Strong |
Widespread |
5D
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High level of expression in entire presumptive retinal pigmented epithelium (RPE) in control eye.
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Specimen
5D':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gas1tm1Fan/Gas1tm1Fan
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup outer layer |
Present |
Regionally restricted |
5D'
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Normal levels of expression in dorsal RPE region; reduced expression in ventral RPE region of mutant eye.
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Specimen
5E:
embryonic day 11.5
(more )
Note: The genetic background of the specimen was either wild type or heterozygous.
Specimen
5E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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The genetic background of the specimen was either wild type or heterozygous.
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Specimen
5E':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gas1tm1Fan/Gas1tm1Fan
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: pigmented retinal epithelium |
Present |
Regionally restricted |
5E'
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Normal levels of expression in dorsal RPE region; reduced expression in ventral RPE region. Authors report subdomains in ventral RPE: domain 2 is closer to midline and has less expression than domain 1 which is further away from midline.
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Specimen
E12.5 not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gas1tm1Fan/Gas1tm1Fan
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Analysis Tools