Antibody:

53BP1 Antibody (A300-272A)

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Weak
TS28: hippocampus	Weak
TS28: ileum	Weak
TS28: lung	Weak
TS28: metanephros	Weak
TS28: cerebral cortex subventricular zone	Weak			The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.
TS28: dentate gyrus subgranular zone	Weak			The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present			Atm-/- mice harbored elevated numbers of Trp53bp1 foci in hippocampus and lung compared to wild type.
TS28: lung	Present			Atm-/- mice harbored elevated numbers of Trp53bp1 foci in hippocampus and lung compared to wild type.
TS28: cerebellum	Weak
TS28: ileum	Weak
TS28: metanephros	Weak
TS28: cerebral cortex subventricular zone	Present			The subventricular zone and subgranular zone had similar positive-foci to that enumerated in the hippocampus.
TS28: dentate gyrus subgranular zone	Present			The subventricular zone and subgranular zone had similar positive-foci to that enumerated in the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present			There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: hippocampus	Present			There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: ileum	Present			There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: lung	Present			There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: metanephros	Present			There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: cerebral cortex subventricular zone	Present			The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.
TS28: dentate gyrus subgranular zone	Present			The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present			High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: hippocampus	Present			High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: lung	Present			High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: metanephros	Present			High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: dentate gyrus subgranular zone	Present
TS28: ileum	Present
TS28: cerebral cortex subventricular zone	Present			The subventricular zone had significantly lower numbers of immunopositive-foci compared to the hippocampus and cerebellum, but the numbers were elevated compared to Lig4<Y288C> mice.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		3B lower , 3B upper

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Ambiguous		3E WT

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		3E Atm-/-

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		3E Lig4Y288C

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present			The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.
TS22: cortical plate	Present			The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present			The level was was not significantly different from wild type.
TS22: cortical plate	Present			The level was was not significantly different from wild type.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present			The level of immunopositive foci was elevated compared to wild type.
TS22: cortical plate	Present			The level of immunopositive foci was elevated compared to wild type.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present			The level of immunopositive foci was elevated compared to wild type.
TS22: cortical plate	Present			The level of immunopositive foci was elevated compared to wild type.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Present			The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.
TS25: cortical plate	Present			The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Present			The level was was not significantly different from wild type.
TS25: cortical plate	Present			The level was was not significantly different from wild type.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Present			The level of immunopositive foci was elevated compared to wild type, but was less than that seen in the Lig4<Y288C> mutant at E14.5.
TS25: cortical plate	Present			The level of immunopositive foci was elevated compared to wild type, but was less than that seen in the Lig4<Y288C> mutant at E14.5.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			Level decreased compared to the level in wild type embryos.
TS28: neocortex	Present			Level decreased compared to the level in wild type embryos.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			Level remained similar to the embryonic level.
TS28: neocortex	Present			Level remained similar to the embryonic level.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present			Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months.
TS28: cerebral cortex subventricular zone	Present			The levels were lower in the subventricular zone than in the isocortex.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present
TS28: neocortex	Present

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			Level remained similar to the embryonic level.
TS28: neocortex	Present			Level remained similar to the embryonic level.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months.
TS28: neocortex	Present			Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			The levels were lower in the subventricular zone than in the isocortex.
TS28: neocortex	Present			Level remained similar to the embryonic level.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			Level decreased compared to the level in wild type embryos.
TS28: neocortex	Ambiguous

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			Level remained similar to the embryonic level.
TS28: neocortex	Present			Level remained similar to the embryonic level.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months. At 2-3 months the level was approximately one-tenth the level at E14.5.
TS28: neocortex	Present			Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months. At 2-3 months the level was approximately one-tenth the level at E14.5.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present			The levels were lower in the subventricular zone than in the isocortex.
TS28: neocortex	Present			Level remained similar to the embryonic level.