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Gene Expression Data
Assay Details
Assay
Reference: J:233378 Barazzuol L, et al., Low levels of endogenous or X-ray-induced DNA double-strand breaks activate apoptosis in adult neural stem cells. J Cell Sci. 2015 Oct 1;128(19):3597-606
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6316062
Gene symbol: Trp53bp1
Gene name: transformation related protein 53 binding protein 1
Results
Specimen adult WT: postnatal adult (more )
Note: This data represents specimens in cell count graphs reported in Fig. 3.
Structure Level Pattern Image Note
TS28: cerebellum Weak
TS28: hippocampus Weak
TS28: ileum Weak
TS28: lung Weak
TS28: metanephros Weak
TS28: cerebral cortex subventricular zone Weak The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.
TS28: dentate gyrus subgranular zone Weak The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.
Specimen adult Atm-/-: postnatal adult; Atmtm1Awb/Atmtm1Awb (more )
Note: This data represents specimens in cell count graphs reported in Fig. 3.
Structure Level Pattern Image Note
TS28: hippocampus Present Atm-/- mice harbored elevated numbers of Trp53bp1 foci in hippocampus and lung compared to wild type.
TS28: lung Present Atm-/- mice harbored elevated numbers of Trp53bp1 foci in hippocampus and lung compared to wild type.
TS28: cerebellum Weak
TS28: ileum Weak
TS28: metanephros Weak
TS28: cerebral cortex subventricular zone Present The subventricular zone and subgranular zone had similar positive-foci to that enumerated in the hippocampus.
TS28: dentate gyrus subgranular zone Present The subventricular zone and subgranular zone had similar positive-foci to that enumerated in the hippocampus.
Specimen adult Lig4Y288C: postnatal adult; Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 3.
Structure Level Pattern Image Note
TS28: cerebellum Present There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: hippocampus Present There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: ileum Present There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: lung Present There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: metanephros Present There was a small, but significant increase in the level of positive foci in Lig4<Y288C> tissues.
TS28: cerebral cortex subventricular zone Present The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.
TS28: dentate gyrus subgranular zone Present The subventricular zone and subgranular zone had similar immunopositive-foci to that enumerated in the cerebellum and hippocampus.
Specimen adult Atm-/-; Lig4Y288C: postnatal adult; Atmtm1Awb/Atmtm1Awb, Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 3.
Structure Level Pattern Image Note
TS28: cerebellum Present High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: hippocampus Present High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: lung Present High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: metanephros Present High levels of Trp53bp1-positive foci were noted in the double mutants.
TS28: dentate gyrus subgranular zone Present
TS28: ileum Present
TS28: cerebral cortex subventricular zone Present The subventricular zone had significantly lower numbers of immunopositive-foci compared to the hippocampus and cerebellum, but the numbers were elevated compared to Lig4<Y288C> mice.
Specimen 3B: postnatal adult; Atmtm1Awb/Atmtm1Awb, Lig4tiny/Lig4tiny (more )
Structure Level Pattern Image Note
TS28: cerebellum Present 3B lower , 3B upper
Specimen 3E WT: postnatal adult (more )
Note: Double labeled: green - Trp53bp1; red - Mki67 (assay MGI:6316334).
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Ambiguous 3E WT
Specimen 3E Atm-/-: postnatal adult; Atmtm1Awb/Atmtm1Awb (more )
Note: Double labeled: green - Trp53bp1; red - Mki67 (assay MGI:6316334).
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present 3E Atm-/-
Specimen 3E Lig4Y288C: postnatal adult; Lig4tiny/Lig4tiny (more )
Note: Double labeled: green - Trp53bp1; red - Mki67 (assay MGI:6316334).
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present 3E Lig4Y288C
Specimen E14.5 WT: embryonic day 14.5 (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.
TS22: cortical plate Present The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.
Specimen E14.5 Atm-/-: embryonic day 14.5; Atmtm1Awb/Atmtm1Awb (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present The level was was not significantly different from wild type.
TS22: cortical plate Present The level was was not significantly different from wild type.
Specimen E14.5 Lig4Y288C: embryonic day 14.5; Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present The level of immunopositive foci was elevated compared to wild type.
TS22: cortical plate Present The level of immunopositive foci was elevated compared to wild type.
Specimen E14.5 Atm-/-; Lig4Y288C: embryonic day 14.5; Atmtm1Awb/Atmtm1Awb, Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Present The level of immunopositive foci was elevated compared to wild type.
TS22: cortical plate Present The level of immunopositive foci was elevated compared to wild type.
Specimen E17.5 WT: embryonic day 17.5 (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS25: cerebral cortex intermediate zone Present The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.
TS25: cortical plate Present The number of immunopositive foci was approximately twofold greater at E14.5 compared to E17.5.
Specimen E17.5 Atm-/-: embryonic day 17.5; Atmtm1Awb/Atmtm1Awb (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS25: cerebral cortex intermediate zone Present The level was was not significantly different from wild type.
TS25: cortical plate Present The level was was not significantly different from wild type.
Specimen E17.5 Lig4Y288C: embryonic day 17.5; Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS25: cerebral cortex intermediate zone Present The level of immunopositive foci was elevated compared to wild type, but was less than that seen in the Lig4<Y288C> mutant at E14.5.
TS25: cortical plate Present The level of immunopositive foci was elevated compared to wild type, but was less than that seen in the Lig4<Y288C> mutant at E14.5.
Specimen P5 WT: postnatal day 5 (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Level decreased compared to the level in wild type embryos.
TS28: neocortex Present Level decreased compared to the level in wild type embryos.
Specimen P5 Atm-/-: postnatal day 5; Atmtm1Awb/Atmtm1Awb (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Level remained similar to the embryonic level.
TS28: neocortex Present Level remained similar to the embryonic level.
Specimen P5 Lig4Y288C: postnatal day 5; Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: neocortex Present Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months.
TS28: cerebral cortex subventricular zone Present The levels were lower in the subventricular zone than in the isocortex.
Specimen P15 WT: postnatal day 15 (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present
TS28: neocortex Present
Specimen P15 Atm-/-: postnatal day 15; Atmtm1Awb/Atmtm1Awb (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Level remained similar to the embryonic level.
TS28: neocortex Present Level remained similar to the embryonic level.
Specimen P15 Lig4Y288C: postnatal day 15; Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months.
TS28: neocortex Present Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months.
Specimen P15 Atm-/-; Lig4Y288C: postnatal day 20; Atmtm1Awb/Atmtm1Awb, Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present The levels were lower in the subventricular zone than in the isocortex.
TS28: neocortex Present Level remained similar to the embryonic level.
Specimen 2/3 months WT: postnatal month 2-3 (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Level decreased compared to the level in wild type embryos.
TS28: neocortex Ambiguous
Specimen 2/3 months Atm-/-: postnatal month 2-3; Atmtm1Awb/Atmtm1Awb (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Level remained similar to the embryonic level.
TS28: neocortex Present Level remained similar to the embryonic level.
Specimen 2/3 months Lig4Y288C: postnatal month 2-3; Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months. At 2-3 months the level was approximately one-tenth the level at E14.5.
TS28: neocortex Present Lig4<Y288C> mice showed a clear decrease in levels from E17.5 to 2-3 months. At 2-3 months the level was approximately one-tenth the level at E14.5.
Specimen 2/3 months Atm-/-; Lig4Y288C: postnatal month 2-3; Atmtm1Awb/Atmtm1Awb, Lig4tiny/Lig4tiny (more )
Note: This data represents specimens in cell count graphs reported in Fig. 4.
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Present The levels were lower in the subventricular zone than in the isocortex.
TS28: neocortex Present Level remained similar to the embryonic level.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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