Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80734
Soto-Suazo M, et al., Differential expression of glycosaminoglycans and proteoglycans in the migratory pathway of the primordial germ cells of the mouse. Histochem Cell Biol. 2002 Jul;118(1):69-78
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6315942
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Gene symbol:
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Sdc4
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Gene name:
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syndecan 4
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Antibody:
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Anti-Syndecan-4
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
4a:
embryonic day 9.5
(more  )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
4a:
(close  )
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Genetic Background:
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involves: A/J * AKR * BALB/c * NZW
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fixative was either 4% paraformaldehyde or methacarn.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: hindgut |
Moderate |
Uniform |
4a
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A moderate, homogeneous immunoreaction was observed in the extracellular matrix.
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Specimen
4b:
embryonic day 10.5
(more )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
4b:
(close )
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Genetic Background:
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involves: A/J * AKR * BALB/c * NZW
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fixative was either 4% paraformaldehyde or methacarn.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindgut dorsal mesentery |
Moderate |
Uniform |
4b
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A moderate, homogeneous immunoreaction was observed in the extracellular matrix.
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Specimen
4c:
embryonic day 11.5
(more )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
4c:
(close )
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Genetic Background:
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involves: A/J * AKR * BALB/c * NZW
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fixative was either 4% paraformaldehyde or methacarn.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: urogenital ridge |
Moderate |
Uniform |
4c
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A moderate, homogeneous immunoreaction was observed in the extracellular matrix.
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Specimen
4d:
embryonic day 12.5
(more )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
4d:
(close )
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Genetic Background:
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involves: A/J * AKR * BALB/c * NZW
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fixative was either 4% paraformaldehyde or methacarn.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: gonad |
Moderate |
Uniform |
4d
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| TS20: hindgut |
Weak |
Regionally restricted |
4d
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Immunostaining was weak in the primordial germ cell migratory route.
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| TS20: hindgut dorsal mesentery |
Weak |
Regionally restricted |
4d
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Immunostaining was weak in the primordial germ cell migratory route.
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| TS20: urogenital ridge |
Weak |
Regionally restricted |
4d
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Immunostaining was weak in the primordial germ cell migratory route.
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Analysis Tools