Gene Expression Data
Assay Details
Assay
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Reference:
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J:80734
Soto-Suazo M, et al., Differential expression of glycosaminoglycans and proteoglycans in the migratory pathway of the primordial germ cells of the mouse. Histochem Cell Biol. 2002 Jul;118(1):69-78
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6315939
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Gene symbol:
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Vcan
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Gene name:
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versican
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Antibody:
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2-B-1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
3a:
embryonic day 9.5
(more )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
3a:
(close )
Genetic Background:
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involves: A/J * AKR * BALB/c * NZW
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fixative was either 4% paraformaldehyde or methacarn.
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Structure |
Level |
Pattern |
Image |
Note |
TS15: hindgut |
Strong |
Uniform |
3a
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Expression was detected in the extracellular matrix.
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TS15: hindgut epithelium |
Strong |
Uniform |
3a
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Expression was detected in the basal membrane.
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Specimen
3b:
embryonic day 10.5
(more )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
3b:
(close )
Genetic Background:
|
involves: A/J * AKR * BALB/c * NZW
|
Age:
|
embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fixative was either 4% paraformaldehyde or methacarn.
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Specimen
3c:
embryonic day 11.5
(more )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
3c:
(close )
Genetic Background:
|
involves: A/J * AKR * BALB/c * NZW
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Age:
|
embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
|
Paraffin
|
Note:
|
Fixative was either 4% paraformaldehyde or methacarn.
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Specimen
3d:
embryonic day 12.5
(more )
Note: Fixative was either 4% paraformaldehyde or methacarn.
Specimen
3d:
(close )
Genetic Background:
|
involves: A/J * AKR * BALB/c * NZW
|
Age:
|
embryonic day 12.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
|
Paraffin
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Note:
|
Fixative was either 4% paraformaldehyde or methacarn.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: gonad |
Strong |
Uniform |
3d
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TS20: hindgut |
Ambiguous |
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3d
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Immunostaining was weak or absent in the primordial germ cell migratory route.
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TS20: hindgut dorsal mesentery |
Ambiguous |
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3d
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Immunostaining was weak or absent in the primordial germ cell migratory route.
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TS20: urogenital ridge |
Ambiguous |
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3d
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Immunostaining was weak or absent in the primordial germ cell migratory route.
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