Probe:	s1p1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: midbrain	Very strong	Regionally restricted	3_s1p1	Expression is adjacent to the mesencephalic vesicle.
TS21: hindbrain	Very strong	Regionally restricted	3_s1p1	Expression is around the 4th ventricle.
TS21: cerebral cortex ventricular layer	Present		3_s1p1
TS21: ganglionic eminence	Present		3_s1p1
TS21: lateral ventricle choroid plexus	Present		3_s1p1
TS21: main olfactory bulb	Present		3_s1p1
TS21: nasal cavity	Present		3_s1p1
TS21: brain ventricular layer	Present	Regionally restricted	3_s1p1	Expression is around the lateral ventricle extending into the hippocampal region.
TS21: telencephalon	Present	Regionally restricted	3_s1p1	Expression is in the hippocampal region.
TS21: brain	Strong	Single cells	3_s1p1	There is punctate expression in scattered cells.
TS21: diencephalon	Strong	Single cells	3_s1p1	There is punctate expression in scattered cells.
TS21: midbrain roof plate	Present	Regionally restricted	3_s1p1	Expression is in a band of cells.
TS21: brain meninges	Present	Regionally restricted	3_s1p1	Expression is between the telencephalon and diencephalon.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present		4A
TS21: ganglionic eminence	Present		4A
TS21: telencephalon	Present	Regionally restricted	4A , 4C	Expression is in the hippocampal region.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present		4B
TS21: midbrain	Present		4D , 4B

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Strong	Regionally restricted	5A	Expression is in the cortical venrticular zone.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong		5C
TS21: cortical preplate	Present	Punctate	5C

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Moderate		5E

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular layer	Trace		5G