Gene Expression Data
Assay Details
Assay
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Reference:
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J:144192
Rajasekharan S, et al., Netrin 1 and Dcc regulate oligodendrocyte process branching and membrane extension via Fyn and RhoA. Development. 2009 Feb;136(3):415-26
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6314649
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Gene symbol:
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Cnp
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Gene name:
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2',3'-cyclic nucleotide 3' phosphodiesterase
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Antibody:
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Anti-CNP
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cnp; red - Nefm (assay MGI:6314650).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: spinal cord |
Present |
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3A
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Expression was detected in premyelinating oligodendrocytes.
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Specimen
3B:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Cnp; red - Nefm (assay MGI:6314650).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: spinal cord |
Present |
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3B
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Expression was detected in premyelinating oligodendrocytes.
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Specimen
3C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1+
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Age:
|
embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Cnp; red - Ntn1 (assay MGI:6314652).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: spinal cord |
Present |
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3C
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Expression was detected in premyelinating oligodendrocytes. Immunopositive cells were adjacent to Ntn1 beta-galactosidase-positive cells in the floor plate.
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Specimen
3D Ntn1+/+:
embryonic day 17.5
(more )
Specimen
3D Ntn1+/+:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 17.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3D Ntn1-/-:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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