Probe:	3.5m
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme	Present		3B
TS16: future brain	Absent		3B

Structure	Level	Pattern	Image	Note
TS17: lung	Present	Regionally restricted	4B/D	Expression was detected in the lung parenchyma.
TS17: main bronchus epithelium	Absent		4B/D
TS17: lung mesenchyme	Weak		4B/D

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present		4G/H
TS21: lung parenchyma	Present		4G/H

Structure	Level	Pattern	Image	Note
TS24: arteriole	Present		4J/L
TS24: lung mesenchyme	Present		4J/L
TS24: lung parenchyma	Present		4J/L
TS24: respiratory system smooth muscle	Present		4J/L
TS24: venule	Present		4J/L
TS24: bronchus epithelium	Absent		4J/L

Structure	Level	Pattern	Image	Note
TS21: larynx	Ambiguous		6B

Structure	Level	Pattern	Image	Note
TS24: epiglottis	Ambiguous		6E/G
TS24: laryngeal cartilage	Ambiguous		6E/G
TS24: perichondrium	Present	Regionally restricted	6E/G	Expression was detected in the perichondrium of the laryngeal cartilages.

Structure	Level	Pattern	Image	Note
TS16: limb mesenchyme	Present		7B
TS16: neural ectoderm	Absent		7B

Structure	Level	Pattern	Image	Note
TS21: perichondrium	Weak	Regionally restricted	7E	Expression was detected in the perichondrium of the long bones.
TS21: cartilage	Weak		7E

Structure	Level	Pattern	Image	Note
TS24: perichondrium	Present	Regionally restricted	7H	Expression was detected in the perichondrium of the long bones.
TS24: cartilage	Present	Regionally restricted	7H	The signal was more evident in the hypertrophic and calcifying zones than other areas of cartilage.
TS24: long bone	Present	Regionally restricted	7H	Signal was detected in the growth plate.

Structure	Level	Pattern	Image	Note
TS24: perichondrium	Present	Regionally restricted	7K	Expression was detected in the perichondrium of flat bones.
TS24: rib cartilage	Present	Regionally restricted	7K	The signal was more evident in the hypertrophic and calcifying zones than other areas of cartilage.

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present	Regionally restricted	8B/C	Expression was most noticeable in the capillaries around joints.
TS24: embryo mesenchyme	Present		8B/C
TS24: ligament	Present		8B/C
TS24: venule	Present		8B/C
TS24: connective tissue	Present	Regionally restricted	8B/C	Expression was detected in tendon.
TS24: skeletal joint	Present	Regionally restricted	8B/C	Expression was detected in cells surrounding ligaments and tendons.

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Weak	Diffuse	9B/D

Structure	Level	Pattern	Image	Note
TS17: atrioventricular endocardial cushion	Present		10C/D
TS17: truncus arteriosus	Strong		10C/D
TS17: endocardial lining	Present	Regionally restricted	10C/D	Expression was restricted mostly to endothelial cells.
TS17: visceral pericardium	Present	Regionally restricted	10C/D	Expression was restricted mostly to the epithelial cells of the epicardium.

Structure	Level	Pattern	Image	Note
TS17: aortic sac	Present		10E/F

Structure	Level	Pattern	Image	Note
TS21: arch of aorta	Strong	Regionally restricted	11B/D	Expression was detected in the intimal and medial cells.
TS21: pulmonary trunk	Strong	Regionally restricted	11B/D	Expression was detected in the intimal and medial cells.
TS21: thymus primordium	Present	Regionally restricted	11B/D	Expression was detected in the capsule and septa of the thymus.

Structure	Level	Pattern	Image	Note
TS24: arch of aorta	Strong
TS24: pulmonary artery	Strong
TS24: sclera	Present