Probe:	mF
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme	Present		3A
TS16: future brain	Absent		3A

Structure	Level	Pattern	Image	Note
TS17: main bronchus epithelium	Strong		4A/C
TS17: lung mesenchyme	Weak	Diffuse	4A/C

Structure	Level	Pattern	Image	Note
TS21: bronchus epithelium	Present	Regionally restricted	4E/F	Expression was detected in the segmental bronchi.
TS21: lung mesenchyme	Present		4E/F

Structure	Level	Pattern	Image	Note
TS24: bronchus epithelium	Absent		4I/K	Expression was not detected in the segmental bronchi at E16.5.
TS24: lung mesenchyme	Present		4I/K
TS24: respiratory system smooth muscle	Present		4I/K
TS24: terminal bronchiole epithelium	Present		4I/K

Structure	Level	Pattern	Image	Note
TS21: larynx	Present	Regionally restricted	6A	Expression was detected in the mesenchymal cells that differentiate into the laryngeal structures.

Structure	Level	Pattern	Image	Note
TS24: laryngeal cartilage	Present	Regionally restricted	6D/F	Expression was detected in the elastic cartilages of the larynx and cuneiform cartilages.
TS24: epiglottis	Present	Regionally restricted	6D/F	Expression was detected in the elastic cartilage of the epiglottis.
TS24: perichondrium	Present	Regionally restricted	6D/F	Expression was detected in the perichondrium of the laryngeal cartilages.

Structure	Level	Pattern	Image	Note
TS16: limb mesenchyme	Present		7A
TS16: neural ectoderm	Absent		7A

Structure	Level	Pattern	Image	Note
TS21: perichondrium	Present	Regionally restricted	7D	Expression was detected in the perichondrium of the long bones.
TS21: cartilage	Weak		7D
TS21: connective tissue	Present	Regionally restricted	7D	Expression was detected in tendon.

Structure	Level	Pattern	Image	Note
TS24: perichondrium	Present	Regionally restricted	7G	Expression was detected in the perichondrium of the long bones.
TS24: cartilage	Weak		7G
TS24: long bone	Present	Regionally restricted	7G	Expression was detected in growth plate.

Structure	Level	Pattern	Image	Note
TS24: perichondrium	Present	Regionally restricted	7J	Expression was detected in the perichondrium of flat bones.
TS24: cartilage	Weak		7J

Structure	Level	Pattern	Image	Note
TS24: ligament	Present		8A
TS24: perichondrium	Present		8A
TS24: connective tissue	Present	Regionally restricted	8A	Expression was detected in tendon.
TS24: cartilage	Present	Regionally restricted	8A	Expression was detected in the periphery of the cartilage.

Structure	Level	Pattern	Image	Note
TS21: main bronchus	Weak		9A/C
TS21: intervertebral disc	Strong	Regionally restricted	9A/C	Intense expression was localized to the peripheral annulus fibrosus.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Uniform	10A/B
TS17: aortic sac	Present		10A/B
TS17: atrioventricular endocardial cushion	Present		10A/B

Structure	Level	Pattern	Image	Note
TS21: arch of aorta	Present	Regionally restricted	11A/C	Expression was observed mostly in the medial layer, particularly its outer portion.
TS21: pulmonary trunk	Present	Regionally restricted	11A/C	Expression was observed mostly in the medial layer, particularly its outer portion.
TS21: cardiac muscle tissue	Present	Uniform	11A/C
TS21: thymus primordium	Present	Regionally restricted	11A/C	Expression was detected in the capsule and septa of the thymus.

Structure	Level	Pattern	Image	Note
TS21: eye	Present	Regionally restricted		Expression was detected in the sclera.