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Gene Expression Data
Assay Details
Assay
Reference: J:275336 Sulistomo HW, et al., Formin homology 2 domain-containing 3 (Fhod3) controls neural plate morphogenesis in mouse cranial neurulation by regulating multidirectional apical constriction. J Biol Chem. 2019 Feb 22;294(8):2924-2934
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6314397
Gene symbol: Myh10
Gene name: myosin, heavy polypeptide 10, non-muscle
Antibody: Anti-Myosin IIB (PRB-445P)
Detection system: Alexa Fluor 555
Assay notes: Fixation was with 3.7% paraformaldehyde.
Results
Specimen 5B +/+ MHC IIB upper: embryonic day 9.5 (more )
Note: Double labeled: red - MHC IIB (Myh10); green - alpha18 (Ctnna1 assay MGI:6314399).
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 5B +/+ upper merge , 5B +/+ upper MHC IIB Expression at apical surface of lateral plate.
Specimen 5B +/+ MHC IIB lower: embryonic day 9.5 (more )
Note: Double labeled: red - MHC IIB (Myh10); green - alpha18 (Ctnna1 assay MGI:6314399).
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 5B +/+ lower MHC IIB , 5B +/+ lower merge Expression at apical surface of lateral plate.
Specimen 5B -/- MHC IIB upper: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: red - MHC IIB (Myh10); green - alpha18 (Ctnna1 assay MGI:6314399).
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present 5B -/- upper merge , 5B -/- upper MHC IIB
Specimen 5B -/- MHC IIB lower: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: red - MHC IIB (Myh10); green - alpha18 (Ctnna1 assay MGI:6314399).
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present 5B -/- lower MHC IIB , 5B -/- lower merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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