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Gene Expression Data
Assay Details
Assay
Reference: J:275336 Sulistomo HW, et al., Formin homology 2 domain-containing 3 (Fhod3) controls neural plate morphogenesis in mouse cranial neurulation by regulating multidirectional apical constriction. J Biol Chem. 2019 Feb 22;294(8):2924-2934
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6314396
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: Anti-ZO-1 (clone ZO1-1A12; 33-9100)
Detection system: Alexa Fluor 488
Assay notes: Fixation was with 3.7% paraformaldehyde.
Results
Specimen 4A +/+ ZO-1 upper: embryonic day 9.5 (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 Present 4A +/+ upper merge , 4A +/+ upper ZO-1
Specimen 4A +/+ ZO-1 lower: embryonic day 9.5 (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 Present 4A +/+ lower merge , 4A +/+ lower Z0-1
Specimen 4A -/- ZO-1 upper: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 Weak Regionally restricted 4A -/- upper ZO-1 , 4A -/- upper merge Authors report expression at convex surface was decreased, although detectable.
Specimen 4A -/- ZO-1 lower: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 Weak Regionally restricted 4A -/- lower merge , 4A -/- lower Z0-1 Authors report expression at convex surface was decreased, although detectable.
Specimen 7B +/+ ZO-1: embryonic day 9.5 (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 7B +/+ ZO-1 , 7B +/+ merge Expression at apical surface of lateral plate.
Specimen 7B -/- ZO-1: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Weak Regionally restricted 7B -/- merge , 7B -/- ZO-1 Expression at apical surface of lateral plate considerably reduced in Fhod3-mull embryos.
Specimen 7D +/+ Z0-1: embryonic day 9.5 (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 7D +/+ Expression at apical surface of lateral plate.
Specimen 7D -/- ZO-1: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 7D -/- Expression at apical surface of lateral plate.
Specimen 7E +/+ ZO-1: embryonic day 9.5 (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 7E +/+ Expression at apical surface of lateral plate.
Specimen 7E +/+ ZO-1 inset: embryonic day 9.5 (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 7E +/+ (inset) Expression at apical surface of lateral plate.
Specimen 7E -/- ZO-1: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 7E -/- Expression at apical surface of lateral plate.
Specimen 7E -/- ZO-1 inset: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 7E -/- (inset) Expression at apical surface of lateral plate.
Specimen 8B +/+ ZO-1: embryonic day 9.5 (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 Present 8B +/+
Specimen 8B -/- ZO-1: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - ZO-1 (Tjp1); red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 Present 8B -/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory