Antibody:	anti-CRABP2
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS16: atrium cardiac muscle	Present		S1c	Evenly distributed.
TS16: primitive ventricle cardiac muscle	Present		S1c	Including the myocardium of the outflow tract. Evenly distributed.
TS16: outflow tract mesenchyme	Present		S1c	Expressed in the myocardium.
TS16: visceral pericardium	Weak		S1c

Structure	Level	Pattern	Image	Note
TS18: atrium cardiac muscle	Strong		S2d	Exhibited a gradient of staining intensity along the myocardium, being the strongest in the outflow tract and atria.
TS18: outflow tract cardiac muscle	Strong		S2d	Exhibited a gradient of staining intensity along the myocardium, being the strongest in the outflow tract and atria.
TS18: primitive ventricle cardiac muscle	Present		S2d
TS18: visceral pericardium	Weak		S2d

Structure	Level	Pattern	Image	Note
TS18: atrium cardiac muscle	Strong		S2e	Exhibited a gradient of staining intensity along the myocardium, being the strongest in the outflow tract and atria.
TS18: outflow tract cardiac muscle	Strong		S2e	Exhibited a gradient of staining intensity along the myocardium, being the strongest in the outflow tract and atria.
TS18: primitive ventricle cardiac muscle	Present		S2e	Exhibited a gradient of staining intensity along the myocardium, being the strongest in the outflow tract and atria.
TS18: visceral pericardium	Weak		S2e

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		S3e	Evenly distributed.
TS20: outflow tract cardiac muscle	Present		S3e	Evenly distributed.
TS20: ventricle cardiac muscle	Present		S3e	Evenly distributed.
TS20: endocardial cushion	Present	Single cells	S3e	Expressed in some cells.
TS20: ventricle endocardial lining	Strong		S3e
TS20: interatrial septum	Present	Regionally restricted	S3e	Expressed in the mesenchymal cap.

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		S3f	Evenly distributed.
TS20: outflow tract cardiac muscle	Present		S3f	Evenly distributed.
TS20: ventricle cardiac muscle	Present		S3f	Evenly distributed.
TS20: ventricle endocardial lining	Strong		S3f
TS20: visceral pericardium	Weak		S3f

Structure	Level	Pattern	Image	Note
TS21: arch of aorta	Present	Uniform	S4e	Homogenous staining.
TS21: atrium cardiac muscle	Present	Uniform	S4e	Homogenous staining.
TS21: outflow tract cardiac muscle	Present	Uniform	S4e	Homogenous staining.
TS21: pulmonary artery	Present	Uniform	S4e	Homogenous staining.
TS21: ventricle cardiac muscle	Present	Uniform	S4e	Homogenous staining.
TS21: ventricle endocardial lining	Strong		S4e
TS21: outflow tract endocardial cushion	Strong	Regionally restricted	S4e	Expressed in the endocardial endothelial cells. Also expressed in some of the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS21: outflow tract cardiac muscle	Present		S4f
TS21: ventricle cardiac muscle	Present		S4f
TS21: outflow tract endocardial cushion	Strong	Regionally restricted	S4f	Expressed in the endocardial endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: heart	Present		S7b
TS22: visceral pericardium	Weak		S7b

Structure	Level	Pattern	Image	Note
TS25: cardiac muscle tissue	Present	Uniform		Evenly distributed.
TS25: great vessel of heart	Absent
TS25: semilunar valve	Absent
TS25: outflow tract	Present	Regionally restricted		Expressed in the mesenchymal cells of the aorto-pulmonary septum.
TS25: atrioventricular valve	Present	Regionally restricted		Expressed in the mesenchyme, localized close to the surface adjacent to the ventricular lumen.
TS25: visceral pericardium	Weak

Structure	Level	Pattern	Image	Note
TS18: interatrial septum	Present	Regionally restricted		Expressed in the mesenchymal cap.

Structure	Level	Pattern	Image	Note
TS20: atrium endocardial lining	Strong

Structure	Level	Pattern	Image	Note
TS21: arch of aorta	Strong			Expressed in the endocardial endothelial cells.
TS21: atrium endocardial lining	Strong			Expressed in the endocardial endothelial cells.
TS21: pulmonary artery	Strong			Expressed in the endocardial endothelial cells.
TS21: semilunar valve	Strong			Expressed in the endocardial endothelial cells.
TS21: aortico-pulmonary spiral septum	Present	Regionally restricted		Expressed in some of the mesenchymal cells.
TS21: visceral pericardium	Weak

Structure	Level	Pattern	Image	Note
TS22: arch of aorta	Strong	Regionally restricted		Expressed in the endocardial endothelial cells.
TS22: atrium endocardial lining	Strong	Regionally restricted		Expressed in the endocardial endothelial cells.
TS22: outflow tract endothelium	Strong	Regionally restricted		Expressed in the endocardial endothelial cells.
TS22: pulmonary artery	Strong	Regionally restricted		Expressed in the endocardial endothelial cells.
TS22: semilunar valve	Strong	Regionally restricted		Expressed in the endocardial endothelial cells.
TS22: atrium cardiac muscle	Present	Uniform		Homogenous staining.
TS22: ventricle cardiac muscle	Present	Uniform		Homogenous staining.
TS22: endocardial cushion	Present	Regionally restricted		Expressed in some of the mesenchymal cells of the outflow tract cushions.
TS22: aortico-pulmonary spiral septum	Present	Regionally restricted		Expressed in some of the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present	Uniform		Evenly distributed.
TS23: great vessel of heart	Absent
TS23: semilunar valve	Absent
TS23: outflow tract	Present	Regionally restricted		Expressed in the mesenchymal cells of the aorto-pulmonary septum.
TS23: atrioventricular valve	Present	Regionally restricted		Expressed in the mesenchyme, localized close to the surface adjacent to the ventricular lumen.
TS23: visceral pericardium	Weak