Gene Expression Data
Assay Details
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Assay
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Reference:
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J:190657
Cho JH, et al., Slits and Robo-2 regulate the coalescence of subsets of olfactory sensory neuron axons within the ventral region of the olfactory bulb. Dev Biol. 2012 Nov 15;371(2):269-79
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6306737
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Gene symbol:
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Or4e5
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Gene name:
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olfactory receptor family 4 subfamily E member 5
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Results
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Specimen
1J:
postnatal day 0; Or4e5tm4Rax/Or4e5 +
(more  )
Note: Double labeled: green - Olfr1507; red - Robo2 (assay MGI:6306738).
Specimen
1J:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Olfr1507; red - Robo2 (assay MGI:6306738).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: main olfactory bulb |
Present |
Regionally restricted |
1K''
,
1J
,
1K'
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Expression was detected in axons in the ventral region of the olfactory bulb.
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Specimen
6A:
postnatal month 3; Or4e5tm4Rax/Or4e5 +
(more )
Note: Control genotype could have included Robo2+/lox, or Omp-cre.
Specimen
6A:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Control genotype could have included Robo2+/lox, or Omp-cre.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
6A
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A beta-galactosidase positive glomerulus was observed in the lateral region of the olfactory bulb.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129 * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Omptm1(cre)Jae/Omp+
Robo2tm1Rilm/Robo2tm1Rilm
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
6B
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A beta-galactosidase positive glomerulus was observed in the lateral region of the olfactory bulb.
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Specimen
6C:
postnatal month 3; Or4e5tm4Rax/Or4e5 +
(more )
Note: Control genotype could have included Robo2+/lox, or Omp-cre.
Specimen
6C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Control genotype could have included Robo2+/lox, or Omp-cre.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
6C
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A second beta-galactosidase positive glomerulus was observed more caudally on the lateral side of the olfactory bulb in 19/29 controls.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129 * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Omptm1(cre)Jae/Omp+
Robo2tm1Rilm/Robo2tm1Rilm
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
6D
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A second beta-galactosidase positive glomerulus was observed more caudally on the lateral side of the olfactory bulb in 13/19 mutants.
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Specimen
6F:
postnatal month 3; Or4e5tm4Rax/Or4e5 +
(more )
Note: Control genotype could have included Robo2+/lox, or Omp-cre.
Specimen
6F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Control genotype could have included Robo2+/lox, or Omp-cre.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
6F
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At least one beta-galactosidase positive glomerulus was observed in the medial region of the olfactory bulb. There were two in 20/26 controls.
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Specimen
6G:
(close )
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Genetic Background:
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involves: 129 * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Omptm1(cre)Jae/Omp+
Robo2tm1Rilm/Robo2tm1Rilm
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
6G
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At least one beta-galactosidase positive glomerulus was observed in the medial region of the olfactory bulb.
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129 * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Omptm1(cre)Jae/Omp+
Robo2tm1Rilm/Robo2tm1Rilm
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
6H
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At least one beta-galactosidase positive glomerulus was observed in the medial region of the olfactory bulb.
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Specimen
7A:
postnatal month 3; Or4e5tm4Rax/Or4e5 +
(more )
Note: Control genotype could have included Slit1+/-.
Specimen
7A:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Control genotype could have included Slit1+/-.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7A
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A beta-galactosidase positive glomerulus was observed in the lateral region of the olfactory bulb.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129 * 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Slit1tm1.1Matl/Slit1tm1.1Matl
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7B
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A beta-galactosidase positive glomerulus was observed in the lateral region of the olfactory bulb.
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Specimen
7C:
postnatal month 3; Or4e5tm4Rax/Or4e5 +
(more )
Note: Control genotype could have included Slit1+/-.
Specimen
7C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Control genotype could have included Slit1+/-.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7C
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A second beta-galactosidase positive glomerulus was observed more caudally on the lateral side of the olfactory bulb in 19/29 controls.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129 * 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Slit1tm1.1Matl/Slit1tm1.1Matl
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7D
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A second beta-galactosidase positive glomerulus was observed more caudally on the lateral side of the olfactory bulb in 11/22 mutants.
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Specimen
7F:
postnatal month 3; Or4e5tm4Rax/Or4e5 +
(more )
Note: Control genotype could have included Slit1+/-.
Specimen
7F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Control genotype could have included Slit1+/-.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7F
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At least one beta-galactosidase positive glomerulus was observed in the medial region of the olfactory bulb. There were two in 20/26 controls.
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Specimen
7G:
(close )
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Genetic Background:
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involves: 129 * 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Slit1tm1.1Matl/Slit1tm1.1Matl
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7G
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At least one beta-galactosidase positive glomerulus was observed in the medial region of the olfactory bulb. There were two in 17/24 mutants.
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Specimen
7H:
postnatal month 3; Or4e5tm4Rax/Or4e5 +
(more )
Note: Control genotype could have included Slit1+/-.
Specimen
7H:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Control genotype could have included Slit1+/-.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7H
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A beta-galactosidase positive glomerulus was observed in the ventromedial region of the olfactory bulb.
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Specimen
7I:
(close )
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Genetic Background:
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involves: 129 * 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Or4e5tm4Rax/Or4e5+
Slit1tm1.1Matl/Slit1tm1.1Matl
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
7I
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A beta-galactosidase positive glomerulus was observed in the ventromedial region of the olfactory bulb. There was a small but significant dorsal shift in the location of the glomerulus in the mutant.
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