Tg(Myh6-Mybpc3*)156Rbns
Transgene Detail
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| Symbol: |
Tg(Myh6-Mybpc3*)156Rbns |
| Name: |
transgene insertion 156, Jeffrey Robbins |
| MGI ID: |
MGI:6304485 |
| Synonyms: |
MyBP-Cdelta9, MyBP-CdeltaCTS |
| Transgene: |
Tg(Myh6-Mybpc3*)156Rbns Location: unknown
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| Alliance: |
Tg(Myh6-Mybpc3*)156Rbns page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Mutation details: The transgene comprises the following elements: the mouse Myh6 promoter, sequence for the human Myc epitope tag immediately after the ATG start codon, the mouse Mybpc3 cDNA with a deletion of the LAGAGRRTS codons (282-290 in SW:Q3UIK0) (the equivalent on peptide level of deletion of TSLAGAGRR (280-288)), and human GH gene poly(A) signal sequence. The deletion of the nine amino-acids in the encoded peptide prevents phosphorylation by protein kinase A and proteolysis by calpain. Three mouse lines (156, 157, 165) were established; line 156 was the highest expressing line (approximately 3.5x endogenous mRNA level) and was used for further experiments.
(J:133394)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:133394 Yang Q, et al., PKA-dependent phosphorylation of cardiac myosin binding protein C in transgenic mice. Cardiovasc Res. 2001 Jul;51(1):80-8 |
| All: |
2 reference(s) |
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Analysis Tools
