About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:149237 Perez-Balaguer A, et al., Shh dependent and independent maintenance of basal midbrain. Mech Dev. 2009 May-Jun;126(5-6):301-13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6304447
Gene symbol: Th
Gene name: tyrosine hydroxylase
Antibody: Anti-Th
Results
Specimen 5A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5A Expression was detected in the dopaminergic cell population.
Specimen 5B: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: midbrain tegmentum Present Regionally restricted 5B Expression was detected in the dopaminergic cells in the ventral tegmental area.
TS26: substantia nigra Present 5B Expression was detected in the dopaminergic cells.
Specimen 5F: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: midbrain Present Scattered 5F The scattered and disorganized immunopositive cells appeared close to the ventral midline.
Specimen 5G: embryonic day 18.5; Shhtm2Amc/Shhtm2Amc, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 5G Immunopositive cells were located close to the floor plate along the midline.
TS26: midbrain mantle layer Present Scattered 5G Only some immunopositive cells were scattered in the surrounding mantle layer.
TS26: midbrain tegmentum Trace Regionally restricted 5G Almost no positive cells were detected in the presumptive area corresponding to the ventral tegmental area.
Specimen not shown: embryonic day 15.5 (more )
Note: Treated with BrdU.
Structure Level Pattern Image Note
TS23: midbrain Present Proliferating dopaminergic cells were counted.
Specimen not shown: embryonic day 15.5; Shhtm2Amc/Shhtm2Amc, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Treated with BrdU.
Structure Level Pattern Image Note
TS23: midbrain Present The number of proliferating dopaminergic cells was dramatically reduced in the conditional mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory