Probe:	PLZF cDNA3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		3A	Expressed in the neuroepithelia of the developing head folds.

Structure	Level	Pattern	Image	Note
TS12: future brain neural fold	Strong	Regionally restricted	3B	Expressed in the anterior edges of the head folds, the rostral extremity of the neural tube formation, and in the region of caudal neuropore.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Strong	Regionally restricted	3C	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS13: future midbrain	Strong	Regionally restricted	3C	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS13: future prosencephalon	Strong	Regionally restricted	3C	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS13: optic pit	Strong		3C

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Strong	Regionally restricted	3D	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS13: future midbrain	Strong	Regionally restricted	3D	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS13: future prosencephalon	Strong	Regionally restricted	3D	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS13: optic pit	Strong		3D
TS13: neural tube floor plate	Absent		3D

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Strong	Regionally restricted	3E	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS14: future midbrain	Strong	Regionally restricted	3E	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS14: prosencephalon	Strong	Regionally restricted	3E	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS14: 1st branchial arch	Strong		3E
TS14: optic pit	Strong		3E
TS14: limb bud	Present		3E
TS14: otic pit	Present		3E
TS14: pronephros	Present		3E
TS14: fronto-nasal process	Present	Regionally restricted	3E	Expressed in frontonasal mesenchyme.
TS14: heart	Absent		3E
TS14: somite	Absent		3E

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong	Regionally restricted	3F	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS17: hindbrain	Strong	Regionally restricted	3F	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS17: midbrain	Strong	Regionally restricted	3F	Strong staining in restricted domains, and lower levels of expression were seen over remaining regions.
TS17: optic cup	Strong		3F
TS17: branchial arch	Present		3F
TS17: fronto-nasal process mesenchyme	Present		3F
TS17: limb bud	Present		3F
TS17: mesonephros	Present		3F
TS17: otocyst	Present		3F
TS17: heart	Absent		3F
TS17: somite	Absent		3F

Structure	Level	Pattern	Image	Note
TS14: rhombomere	Present	Regionally restricted	4A	Down-regulation of expression in the central regions encompassing rhombomere 3 (r3) and r5.
TS14: rhombomere 4	Present		4A
TS14: floor plate	Absent		4A

Structure	Level	Pattern	Image	Note
TS14: rhombomere	Present	Regionally restricted	4B	Down-regulation of expression in the central regions encompassing rhombomere 3 (r3) and r5.
TS14: floor plate	Absent		4B

Structure	Level	Pattern	Image	Note
TS16: rhombomere	Strong	Regionally restricted	4C	Down-regulation of expression also became apparent in the even-numbered rhombomeres, and strong staining at the boundaries were observed in all the rhombomeres.
TS16: neural tube	Strong	Regionally restricted	4C	High levels of expression remain in other anterior and posterior regions.
TS16: floor plate	Absent		4C

Structure	Level	Pattern	Image	Note
TS14: floor plate	Absent
TS14: notochord	Absent

Structure	Level	Pattern	Image	Note
TS17: floor plate	Absent
TS17: notochord	Absent