Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:47986
Dellovade TL, et al., The gonadotropin-releasing hormone system does not develop in Small-Eye (Sey) mouse phenotype. Brain Res Dev Brain Res. 1998 May 15;107(2):233-40
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6303851
|
Gene symbol:
|
Gnrh1
|
Gene name:
|
gonadotropin releasing hormone 1
|
Antibody:
|
LR1
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Results
|
Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
1A:
embryonic day 14.5
(more )
Note: Double labeled: red/brown - Gnrh1; blue/gray - Ncam1 (assay MGI:6303885).
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red/brown - Gnrh1; blue/gray - Ncam1 (assay MGI:6303885).
|
Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: forebrain |
Present |
Regionally restricted |
|
Thick immunoreactive cords were entering the forebrain.
|
Specimen
1C:
embryonic day 16.5
(more )
Note: Double labeled: red/brown - Gnrh1; blue/gray - Ncam1 (assay MGI:6303885).
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red/brown - Gnrh1; blue/gray - Ncam1 (assay MGI:6303885).
|
Specimen
1E:
embryonic day 18.5
(more )
Specimen
1E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: nose |
Absent |
|
|
|
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
3 E13 wt:
embryonic day 13.5
(more )
Specimen
3 E13 wt:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
3 E13 Sey/+:
embryonic day 13.5; Pax6Sey/Pax6 +
(more )
Specimen
3 E13 Sey/+:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: nasal septum |
Present |
|
|
Expression was similar to wild type.
|
TS21: olfactory epithelium |
Present |
|
|
Expression was similar to wild type.
|
TS21: forebrain |
Present |
Regionally restricted |
|
Expression was in cells traversing from the periphery into the developing forebrain as in wild type.
|
Specimen
3 E15 wt:
embryonic day 15.5
(more )
Specimen
3 E15 wt:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
3 E15 Sey/+:
embryonic day 15.5; Pax6Sey/Pax6 +
(more )
Specimen
3 E15 Sey/+:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6+
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
3 E18 wt:
embryonic day 18.5
(more )
Specimen
3 E18 wt:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
Specimen
3 E18 Sey/+:
embryonic day 18.5; Pax6Sey/Pax6 +
(more )
Specimen
3 E18 Sey/+:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Pax6Sey/Pax6+
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Embedding:
|
Paraffin
|
|