Probe:	Sox6 cDNA10
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Graded	4A	Expression was highest in the forebrain gradually decreasing in a rostrocaudal gradient to a region posterior to the forelimb bud, beyond which expression ceased. No dorsoventral gradient was visible.
TS15: otic pit	Present		4A
TS15: embryo mesoderm	Absent		4A
TS15: surface ectoderm	Absent		4A

Structure	Level	Pattern	Image	Note
TS15: ventricular layer	Present	Regionally restricted	4B	Expression was detected in the ventricular zone of the midbrain, hindbrain and upper spinal cord, diminishing posterior to the forelimb buds.
TS15: otic pit	Present		4B

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Graded	4C	Expression was highest in the forebrain gradually decreasing in a rostrocaudal gradient to a point in the upper cervical region. No dorsoventral gradient was visible.
TS17: ventricular layer	Present	Regionally restricted	4C	Expression was detected in the ventricular zone of the brain and spinal cord.
TS17: otocyst	Present		4C
TS17: embryo mesoderm	Absent		4C
TS17: surface ectoderm	Absent		4C

Structure	Level	Pattern	Image	Note
TS17: ventricular layer	Present	Regionally restricted	4D	Expression was detected in the ventricular zone of the brain and spinal cord.
TS17: 4th ventricle	Present		4D
TS17: hindbrain	Present		4D
TS17: midbrain	Present		4D
TS17: optic stalk	Present		4D
TS17: otocyst	Present		4D
TS17: telencephalon	Present		4D
TS17: embryo mesoderm	Absent		4D
TS17: surface ectoderm	Absent		4D

Structure	Level	Pattern	Image	Note
TS20: brain	Absent
TS20: spinal cord	Weak