Probe:	Igfbp2 cDNA4
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS16: embryo ectoderm	Strong	Graded	2A	The highest levels of expression were found rostrally and fading out caudally.
TS16: forelimb bud apical ectodermal ridge	Present		2A
TS16: hindlimb bud	Present	Diffuse	2A

Structure	Level	Pattern	Image	Note
TS16: forelimb bud apical ectodermal ridge	Strong		2B
TS16: forelimb bud	Strong	Regionally restricted	2B	Expression was detected in a central proximal region.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud apical ectodermal ridge	Strong		2C

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud ectoderm	Weak	Regionally restricted	2D	Expression was weaker in the hindlimb apical ectodermal region.
TS16: hindlimb bud	Present	Diffuse	2D	Expression was more diffuse and did not extend around the entire margin of the bud.

Structure	Level	Pattern	Image	Note
TS18: embryo	Strong	Non-Uniform	2E	Expression was found in both the rostral and caudal region, but expression was higher in the rostral region.
TS18: apical ectodermal ridge	Present		2E
TS18: forelimb bud	Present	Regionally restricted	2E	Proximal chevron expression pattern in forelimb bud.

Structure	Level	Pattern	Image	Note
TS18: limb bud	Present	Regionally restricted	2F	Proximal expression was found on the dorsal surface.

Structure	Level	Pattern	Image	Note
TS18: limb bud	Present	Regionally restricted	2G	Proximal expression was found on the dorsal surface.

Structure	Level	Pattern	Image	Note
TS18: apical ectodermal ridge	Present		2H

Structure	Level	Pattern	Image	Note
TS18: apical ectodermal ridge	Present		2I
TS18: limb ectoderm	Present	Regionally restricted	2I	Expression was detected in dorsal ectoderm.
TS18: limb mesenchyme	Present	Regionally restricted	2I	Expression was detected in subectodermal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: embryo ectoderm	Present	Uniform	2J
TS20: limb interdigital region	Present	Regionally restricted	2J	Expression was detected in the distal interdigital region.

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		2K
TS21: hindbrain	Present		2K
TS21: limb	Present		2K
TS21: midbrain	Present		2K
TS21: tail	Present		2K

Structure	Level	Pattern	Image	Note
TS21: forearm	Present	Regionally restricted	2L	Expression was seen in the dorsal view of the zeugopod.
TS21: forelimb interdigital region	Present	Regionally restricted	2L	Expression was detected in the distal interdigital region.
TS21: hand	Present	Regionally restricted	2L	Expression was detected in the digit flanking region.

Structure	Level	Pattern	Image	Note
TS21: forearm	Present	Regionally restricted	2M	The ventral view of the zeugopod showed no expression. Expression was only found dorsally.

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Regionally restricted	2N	Expression was detected in the apical ectodermal ridge at the tips of the digits, but not between digits.

Structure	Level	Pattern	Image	Note
TS22: face	Present	Regionally restricted	2O	Expression was detected in snout.
TS22: vibrissa	Present	Regionally restricted	2O	Expression was detected at the base of the whisker barrels.
TS22: tail	Present		2O
TS22: limb	Present	Regionally restricted	2O	Expression was detected in distal regions and not in the proximal region.

Structure	Level	Pattern	Image	Note
TS22: hindlimb mesenchyme	Present	Regionally restricted	2P	Expression was detected in mesenchyme lateral to the cartilage forming region.
TS22: hindlimb digit	Present	Regionally restricted	2P	Expression was localized in the digit ectoderm.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Absent		3A
TS19: limb	Present	Regionally restricted	3A	Expression was detected in the proximal aspect in a chevron pattern.
TS19: embryo	Present	Regionally restricted	3A	Expression in the rostral aspect of the embryo was normal.

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region	Absent		3B

Structure	Level	Pattern	Image	Note
TS21: head	Present		3C
TS21: tail	Present		3C
TS21: limb	Present	Regionally restricted	3C	Expression was detected in the posterior distal region.

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	3D	Expression was detected in the posterior distal region.

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	3E	Expression was detected in mesenchyme and ectoderm of the distal region. Levels in dorsal ectoderm were higher than in ventral ectoderm.
TS21: hindlimb mesenchyme	Present	Regionally restricted	3E	Expression was detected in mesenchyme and ectoderm of the distal region. Levels in dorsal ectoderm were higher than in ventral ectoderm.

Structure	Level	Pattern	Image	Note
TS22: face	Present	Regionally restricted	3F	Expression was detected in the snout.
TS22: tail	Present		3F
TS22: forelimb	Present	Regionally restricted	3F	Expression was detected in the distal region.
TS22: hindlimb	Present	Regionally restricted	3F	Expression was detected in the distal region.