Probe:	Igf1 cDNA7
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS19: fronto-nasal process	Present		1A
TS19: trunk	Strong	Regionally restricted	1A	Expression was detected in the flank region and posterior lateral tissue.
TS19: branchial arch	Present	Regionally restricted	1A	Expression was detected in the distal portions of the branchial arches.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	1B	Expression was detected in the proximal part of the forelimb bud. Expression was stronger in the anterior compared to posterior.

Structure	Level	Pattern	Image	Note
TS20: foot	Present		1C
TS20: hand	Present		1C

Structure	Level	Pattern	Image	Note
TS21: forearm	Present		1D
TS21: hand	Present	Regionally restricted	1D	Expression was maintained adjacent to digits in the autopod.

Structure	Level	Pattern	Image	Note
TS22: wrist	Present		1E

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	1F	Expression was detected on either side of the developing digits.
TS22: forearm	Present	Regionally restricted	1F	Expression was detected on lateral edges of zeugopodal elements.

Structure	Level	Pattern	Image	Note
TS19: fronto-nasal process	Present	Regionally restricted	1G	Expression was predominantly in the ventral frontal nasal process.
TS19: trunk	Present	Regionally restricted	1G	Normal expression was seen in the flank region.
TS19: forelimb bud	Present	Regionally restricted	1G	Anterior proximal expression was not strong in forelimb bud.

Structure	Level	Pattern	Image	Note
TS19: fronto-nasal process	Weak	Widespread	1H
TS19: forelimb bud	Trace	Regionally restricted	1H	Expression in anterior proximal forelimb bud was almost absent.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Trace		1I

Structure	Level	Pattern	Image	Note
TS21: forearm	Present	Regionally restricted	1J	A band of expression was detected in the zeugopod.
TS21: hand	Present	Regionally restricted	1J	Expression was only in the anterior region of forelimb autopod.

Structure	Level	Pattern	Image	Note
TS22: zeugopod	Present	Regionally restricted	1K	Expression was upregulated in posterior zeugopod.
TS22: autopod	Present	Regionally restricted	1K	Expression in autopod extends to the distal extremity.
TS22: limb mesenchyme	Present	Regionally restricted	1K	Expression was found throughout the zeugopod, but was considerably upregulated in mesenchymal tissues of the autopod.

Structure	Level	Pattern	Image	Note
TS22: zeugopod	Present	Regionally restricted	1L	Expression was upregulated in posterior zeugopod.
TS22: autopod	Present	Regionally restricted	1L	Expression in autopod extends to the distal extremity.
TS22: limb mesenchyme	Present	Regionally restricted	1L	Expression was found throughout the zeugopod, but was considerably upregulated in mesenchymal tissues of the autopod.

Structure	Level	Pattern	Image	Note
TS21: hand	Strong	Regionally restricted	1M	Expression in the forelimb autopod was detected lateral to condensing cartilage.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	1N	Expression was detected throughout the mesenchyme adjacent to one condensing cartilage element in the autopod.