Gene Expression Data
Assay Details
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Assay
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Reference:
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J:77376
Hirata T, et al., Mosaic development of the olfactory cortex with Pax6-dependent and -independent components. Brain Res Dev Brain Res. 2002 May 30;136(1):17-26
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6297065
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Gene symbol:
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Rtn1
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Gene name:
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reticulon 1
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Antibody:
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mAb 9-4c
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
1D:
embryonic day 12.5
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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CBA
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Strong |
Regionally restricted |
1D
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Expressed in radial fibers projecting from the ventricular zone between the pallio-subpallial boundary and morphological sulcus. The distal ends of positive radial fibers were connected to a more dorsal part of the olfactory cortex. Also expressed in the septum.
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| TS20: lateral ganglionic eminence |
Present |
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1D
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Occasionally stained in the radial fibers. Staining intensity was weaker than that of the pallio-subpallial boundary.
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| TS20: medial ganglionic eminence |
Present |
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1D
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Occasionally stained in the radial fibers. Staining intensity was weaker than that of the pallio-subpallial boundary.
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| TS20: preoptic area |
Strong |
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1D
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Specimen
1F:
embryonic day 14.5
(more )
Note: Double labeled: red - Rtn1; green - Pax6 (assay MGI:6297063).
Specimen
1F:
(close )
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Genetic Background:
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CBA
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rtn1; green - Pax6 (assay MGI:6297063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
1F
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Expressed in radial fibers projecting from the pallio-subpallial boundary. Poistive fibers were more twisted, and formed a glial palisade dividing the pallium from the subpallium. A great number of positive cell bodies were associated with the distal parts of positive radial fibers.
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| TS22: olfactory cortex |
Present |
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1F
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Expressed in radial fibers, the distal ends of which fanned out and covered a wide area of the olfactory cortex.
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Specimen
1G:
embryonic day 14.5
(more )
Note: Double labeled: red - Rtn1; green - Pax6 (assay MGI:6297063).
Specimen
1G:
(close )
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Genetic Background:
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CBA
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rtn1; green - Pax6 (assay MGI:6297063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
1G
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Expressed in radial fibers, mainly projected from the edge of the Pax6-positive ventricular zone.
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Specimen
1H:
embryonic day 14.5
(more )
Note: Double labeled: red - Rtn1; green - Pax6 (assay MGI:6297063).
Specimen
1H:
(close )
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Genetic Background:
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CBA
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rtn1; green - Pax6 (assay MGI:6297063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: olfactory cortex |
Present |
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1H
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Expressed in radial fibers, which constructed the deep layers in the piriform cortex.
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Specimen
4A:
embryonic day 14.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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CBA
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4B:
(close )
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Genetic Background:
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involves: CBA
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools