Antibody:	anti-Serca2a
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Fixative was: 40% methanol: 40% acetone: 20% water.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Strong		1d

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		3a

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present		3c

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		3e

Structure	Level	Pattern	Image	Note
TS23: heart septum	Weak	Regionally restricted	3g	Expression is in cardiomyocytes.

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		4c
TS19: sinus venosus	Present		4c

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		4e
TS21: left superior vena cava	Present		4e
TS21: right superior vena cava	Present		4e

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present		4g
TS22: left superior vena cava	Present	Regionally restricted	4g	Expression is in the proximal part of the wall.

Structure	Level	Pattern	Image	Note
TS20: sinus venosus right horn	Present		5a
TS20: dorsal mesocardium	Weak		5a

Structure	Level	Pattern	Image	Note
TS19: dorsal mesocardium	Present		5c
TS19: heart	Present	Regionally restricted	5c	Expression is in the spina vestibuli.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Regionally restricted	5e	Expression is in the dorsal mesocardium and spina vestibuli.

Structure	Level	Pattern	Image	Note
TS21: septum primum	Present	Regionally restricted	6a	Expression is at the caudal tip.

Structure	Level	Pattern	Image	Note
TS21: interventricular septum muscular part	Present	Regionally restricted	6c	Expression is at the cranial tip.
TS21: atrioventricular endocardial cushion	Present	Regionally restricted	6c	Expression is in the mesenchyme.
TS21: heart	Present	Regionally restricted	6c	Expression is in the mesenchyme adjacent to the atrioventricular cushion mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Strong		6e

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Strong		6g
TS22: septum primum	Present	Regionally restricted	6g	Expression is at the caudal tip.

Structure	Level	Pattern	Image	Note
TS19: dorsal mesocardium	Weak	Regionally restricted	7c	Expression is in the pulmonary vein.
TS19: vein	Weak	Regionally restricted	7c	Expression is in the pulmonary vein.

Structure	Level	Pattern	Image	Note
TS21: dorsal mesocardium	Weak	Regionally restricted	7e	Expression is in the pulmonary vein and is absent further in the direction towards the lungs.
TS21: heart	Weak	Regionally restricted	7e	Expression is in the pulmonary vein and is absent further in the direction towards the lungs.
TS21: vein	Weak	Regionally restricted	7e	Expression is in the pulmonary vein and is absent further in the direction towards the lungs.

Structure	Level	Pattern	Image	Note
TS22: vein	Weak	Scattered	7g	Expression is in the pulmonary vein at the level of the first bifurcation.