Gene Expression Data
Assay Details
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Assay
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Reference:
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J:48563
Sporle R, et al., Paradox segmentation along inter- and intrasomitic borderlines is followed by dysmorphology of the axial skeleton in the open brain (opb) mouse mutant. Dev Genet. 1998;22(4):359-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6295998
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Gene symbol:
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Pax9
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Gene name:
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paired box 9
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Probe:
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Pax9 cDNA7
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4G:
embryonic day 9.5-10.0
(more  )
Note: TS15-16
Specimen
4G:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5-10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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TS15-16
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Strong |
Regionally restricted |
4G
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Expression was more clearly restricted to stripes along the caudal and rostral sclerotome borders. Narrow stripes of expression along the rostral borders of mature thoracic sclerotomes.
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Specimen
4H:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 9.5-10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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TS15-16
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Present |
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4H
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Expression was not obviously up-regulated in the rostral sclerotome halves despite the putative defect of the rostro-caudal polarities in the sclerotomes. No expression along the rostral borders of mature thoracic sclerotomes. Many somite borders already became indistinct.
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Specimen
4I:
embryonic day 10.5
(more )
Note: TS17
Specimen
4I:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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TS17
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: sclerotome |
Strong |
Regionally restricted |
4I
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Expression was more clearly restricted to stripes along the caudal and rostral sclerotome borders.
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Specimen
4K:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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TS17
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: sclerotome |
Present |
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4K
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Expression was not obviously up-regulated in the rostral sclerotome halves despite the putative defect of the rostro-caudal polarities in the sclerotomes. Expression along the rostral borders of the sclerotomes was still weak or not detectable in thoracic sclerotomes.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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TS15
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Present |
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Expression was weaker in the caudal sclerotome halves, but not in the branchial furrows.
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Analysis Tools
