Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:48563
Sporle R, et al., Paradox segmentation along inter- and intrasomitic borderlines is followed by dysmorphology of the axial skeleton in the open brain (opb) mouse mutant. Dev Genet. 1998;22(4):359-73
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6295978
|
|
Gene symbol:
|
Pax1
|
|
Gene name:
|
paired box 1
|
|
Probe:
|
Pax1 cDNA-4
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4A:
embryonic day 9.5
(more  )
Note: TS15
Specimen
4A:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Paraformaldehyde
|
|
Note:
|
TS15
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Strong |
Regionally restricted |
4A
|
Expressed in the central sclerotomes of the mature rostral somites as stripes along the rostral and caudal somite borders. In younger somites, expressed more evenly throughout the central sclerotome moieties. Clearly respected the somite borders. Expression was evenly distributed across the borderlines.
|
Specimen
4B:
(close )
|
Genetic Background:
|
involves: C57BL/6 * NMRI
|
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Paraformaldehyde
|
|
Note:
|
TS15
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Present |
Uniform |
4B
|
Remain more evenly distributed also throughout the mature sclerotomes. Borders between adjacent sclerotomes were not always distinct. Expression appeared to be downregulated along many of the intrasomitic borderlines.
|
Specimen
4C:
embryonic day 9.5
(more )
Note: TS15
Specimen
4C:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Paraformaldehyde
|
|
Note:
|
TS15
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Strong |
Regionally restricted |
4C
|
Expressed in the central sclerotomes of the mature rostral somites as stripes along the rostral and caudal somite borders. Clearly respected the somite borders. Expression was evenly distributed across the borderlines.
|
Specimen
4D:
(close )
|
Genetic Background:
|
involves: C57BL/6 * NMRI
|
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Paraformaldehyde
|
|
Note:
|
TS15
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Present |
Uniform |
4D
|
Remain more evenly distributed also throughout the mature sclerotomes. Borders between adjacent sclerotomes were not always distinct. Expression appeared to be downregulated along many of the intrasomitic borderlines.
|
Specimen
4E:
embryonic day 12.5
(more )
Note: TS21
Specimen
4E:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
TS21
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
Regionally restricted |
4E
|
Expression was maintained longest at the levels of the intrasomitic fissures, where the Pax1-expressing intervertebral disc anlagen develop later.
|
Specimen
4F:
(close )
|
Genetic Background:
|
involves: C57BL/6 * NMRI
|
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
TS21
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: axial skeleton |
Present |
Regionally restricted |
4F
|
Expression was restricted to the marginal zones of the thoracic intervertebral disc anlagen as normally only observed for the anlagen of the chondrifying vertebral bodies. Less frequently, expressed in a stripe along the plane of bilateral symmetry.
|
|
Analysis Tools