Probe:	Tbeta4 cDNA
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		2A/B
TS15: trigeminal preganglion	Present		2A/B
TS15: diencephalon	Present	Regionally restricted	2A/B	Expression is in the future thalamus and is low in the future hypothalamus.
TS15: future brain floor plate	Weak		2A/B
TS15: optic cup	Weak		2A/B
TS15: optic stalk	Weak		2A/B
TS15: head mesenchyme	Present	Regionally restricted	2A/B	Expression is in vascularized areas.
TS15: neural tube	Present	Regionally restricted	2A/B	Expression in the rostral spinal cord is higher in the basal plate than in the alar plate.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	2C , 2E/F	Expression is ventral.
TS20: brain mantle layer	Strong		2E/F , 2C
TS20: corpus striatum	Strong		2E/F , 2C
TS20: facial ganglion	Strong		2E/F , 2C
TS20: glossopharyngeal ganglion	Strong		2E/F , 2C
TS20: thalamus	Strong		2E/F , 2C
TS20: trigeminal ganglion	Strong		2E/F , 2C
TS20: vagus ganglion	Strong		2E/F , 2C
TS20: vestibulo-cochlear ganglion	Strong		2E/F , 2C
TS20: brain vascular element	Strong		2C , 2E/F
TS20: brain	Strong	Single cells	2E/F , 2C	Expression is in Kolmer cells.
TS20: brainstem	Present		2C
TS20: diencephalon	Present		2C
TS20: lateral ventricle	Present		2C
TS20: brain ventricular layer	Present		2E/F , 2C
TS20: hypothalamus	Weak	Regionally restricted	2C , 2E/F

Structure	Level	Pattern	Image	Note
TS20: brainstem	Present		2D
TS20: spinal cord	Present		2D
TS20: spinal ganglion	Present		2D
TS20: skeletal muscle	Present	Regionally restricted	2D	Expression is in the vertebral axis musculature.
TS20: trunk musculature	Moderate		2D
TS20: sclerotome	Weak		2D
TS20: vertebral cartilage condensation	Absent		2D

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Strong		2G/H
TS20: spinal ganglion	Strong		2G/H
TS20: brain meninges	Strong	Regionally restricted	2G/H	Expression is in meningeal blood vessels.
TS20: brain vascular element	Strong	Regionally restricted	2G/H	Expression is in meningeal blood vessels.
TS20: spinal cord	Present	Graded	2G/H	There is a gradient of expression from the basal to the alar plate.
TS20: brain ventricular layer	Present		2G/H

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle	Present	Regionally restricted	2I/J	Expression is Kolmer cells in the roof of the 4th ventricle.
TS20: brain roof plate	Present	Regionally restricted	2I/J	Expression is Kolmer cells in the roof of the 4th ventricle.
TS20: brain meninges	Present	Regionally restricted	2I/J	Expression is in meningeal blood vessels.
TS20: brain vascular element	Present	Regionally restricted	2I/J	Expression is in meningeal blood vessels.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present
TS15: somite	Weak