Srd5a3tm1c(EUCOMM)Wtsi
Targeted Allele Detail
|
Symbol: |
Srd5a3tm1c(EUCOMM)Wtsi |
Name: |
steroid 5 alpha-reductase 3; targeted mutation 1c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:6295430 |
Synonyms: |
Srd5a3fl |
Gene: |
Srd5a3 Location: Chr5:76288118-76303351 bp, + strand Genetic Position: Chr5, 40.5 cM
|
Alliance: |
Srd5a3tm1c(EUCOMM)Wtsi page
|
IMPC: |
Srd5a3 gene page |
|
Germline Transmission: |
Earliest citation of germline transmission:
J:267496
|
Parent Cell Line: |
JM8A3.N1 (ES Cell)
|
Strain of Origin: |
C57BL/6N-Atm1Brd
|
Project Collection: |
EUCOMM
|
|
Allele Type: |
|
Targeted (Conditional ready) |
Mutation: |
|
Insertion
|
|
|
Mutation details: The L1L2_Bact_P cassette was inserted at position 76295065 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 76298062. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:267496)
|
|
|
|
Original: |
J:267496 Medina-Cano D, et al., High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect. Elife. 2018 Oct 12;7:e38309 |
All: |
1 reference(s) |
|