Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(Gfap-flpo)62Thara
Name:	transgene insertion 62, Toshiro Hara
MGI ID:	MGI:6294098
Synonyms:	GFAP-FLPo
Transgene:	Tg(Gfap-flpo)62Thara  Location: unknown
Alliance:	Tg(Gfap-flpo)62Thara page

Strain of Origin:

B6D2F1

Transgene Type:    Transgenic (Recombinase) Mutation:    Insertion   Tg(Gfap-flpo)62Thara expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Gfap (MGI:95697)   Mutation details: A transgene consist of an 15 kbp mouse glial fibrillary acidic protein (Gfap) sequence including its promoter region, regulatory elements, exons, introns and 3' UTR, as well as having a point mutation (ATG to TTG) in the Gfap start codon site to prevent GFAP expression from the transgene with an optimized FLP recombinase (flpo) followed by BGH polyA integrated into the Gfap first exon was used. The final transgene was used for pronuclear injection into B6D2F1/J x B6D2F1/J embryos. Founder animals were bred to C57BL/6J inbred mice for germline transmission. Founder line #62 was identified to have flpo expression predominantly in brain, and had the least amount of non-brain expression. (J:278165)

Activity:	Tissue activity of this recombinase allele
Driver:	Gfap (mouse) Summary of all recombinase alleles driven by Gfap. Comparative matrix view of recombinase activities Alliance mouse Gfap gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:278165 Hara T, et al., Modeling Gliomas Using Two Recombinases. Cancer Res. 2019 Aug 1;79(15):3983-3991
All:	1 reference(s)