Tg(Gfap-flpo)62Thara
Transgene Detail
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Symbol: |
Tg(Gfap-flpo)62Thara |
Name: |
transgene insertion 62, Toshiro Hara |
MGI ID: |
MGI:6294098 |
Synonyms: |
GFAP-FLPo |
Transgene: |
Tg(Gfap-flpo)62Thara Location: unknown
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Alliance: |
Tg(Gfap-flpo)62Thara page
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Transgene Type: |
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Transgenic (Recombinase) |
Mutation: |
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Insertion
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Tg(Gfap-flpo)62Thara expression driven by
1 gene
Transgene expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Gfap (MGI:95697) |
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Mutation details: A transgene consist of an 15 kbp mouse glial fibrillary acidic protein (Gfap) sequence including its promoter region, regulatory elements, exons, introns and 3' UTR, as well as having a point mutation (ATG to TTG) in the Gfap start codon site to prevent GFAP expression from the transgene with an optimized FLP recombinase (flpo) followed by BGH polyA integrated into the Gfap first exon was used. The final transgene was used for pronuclear injection into B6D2F1/J x B6D2F1/J embryos. Founder animals were bred to C57BL/6J inbred mice for germline transmission. Founder line #62 was identified to have flpo expression predominantly in brain, and had the least amount of non-brain expression.
(J:278165)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Gfap
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:278165 Hara T, et al., Modeling Gliomas Using Two Recombinases. Cancer Res. 2019 Aug 1;79(15):3983-3991 |
All: |
1 reference(s) |
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