Tg(Sftpc-GCaMP8)B23-5Mik
Transgene Detail
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| Symbol: |
Tg(Sftpc-GCaMP8)B23-5Mik |
| Name: |
transgene insertion B23-5, Michael I Kotlikoff |
| MGI ID: |
MGI:6294087 |
| Synonyms: |
B23-SftpcBAC-GCaMP8, SP-C-GCaMP8 |
| Transgene: |
Tg(Sftpc-GCaMP8)B23-5Mik Location: unknown
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| Alliance: |
Tg(Sftpc-GCaMP8)B23-5Mik page
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| Strain of Origin: |
FVB/N X B6(Cg)-Tyrc-2J/J
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The ~180.5 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-247J9 was obtained; containing the entire surfactant associated protein C locus (Sftpc) as well as ~70 kbp of centromeric flanking sequences (including the complete loci Phyhip and Bmp1) and ~108 kbp of telomeric flanking sequences (including the complete loci Lgi3, Reep4, Hr, Nudt18, Fam160b2 and Nudt18, as well as a 3' portion of Dmtn). Using homologous recombination/BAC recombineering, a 2230 bp GCaMP8-SV40pA construct was inserted into the ATG start site of the BAC Sftpc gene. No other loci on the BAC were altered. The 11.6 kbp BAC vector (pBACe3.6) contained a chloramphenicol selection cassette. The genetically encoded calcium indicator GCaMP8 is a sensitive GCaMP variant with high dynamic range and fast response kinetics. Founder line B23-5 was identified with high sensor expression in pulmonary type II alveolar epithelial cells.
(J:314125)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858 |
| All: |
1 reference(s) |
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Analysis Tools
