Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Hcn4-COP4*L132C,-lacZ)B17-2Mik
Name:	transgene insertion B17-2, Michael I Kotlikoff
MGI ID:	MGI:6294085
Synonyms:	B17-Hcn4BAC-CatCh2_IRES_lacZ, HCN4-CatCh-IRES-lacZ
Transgene:	Tg(Hcn4-COP4*L132C,-lacZ)B17-2Mik  Location: unknown
Alliance:	Tg(Hcn4-COP4*L132C,-lacZ)B17-2Mik page

Strain of Origin:

FVB/N X B6(Cg)-Tyrc-2J/J

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The ~237 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-281H22 was obtained; containing the entire hyperpolarization-activated, cyclic nucleotide-gated K+ 4 locus (Hcn4), as well as ~66 kbp centromeric flanking sequences and ~134 kbp telomeric flanking sequences (including a 3' portion of the Neo1 locus). Using homologous recombination/BAC recombineering, a 5640 bp CatCh-IRES-lacZ-pA construct was inserted into the ATG start site of the BAC Hcn4 gene (replacing the initiation codon of Hcn4 in exon 1). No other loci on the BAC were altered. The 11.6 kbp BAC vector (pBACe3.6) contained a chloramphenicol selection cassette. The calcium translocating channelrhodopsin CatCh [ChR2(L132C)] cDNA sequence used here encodes the first 309 amino acids of green alga Chlamydomonas reinhardtii channelrhodopsin-2 (ChR2 or cop4) that has been modified with an L132C amino acid substitution (CTC-to-TGC) designed to cause enhanced Ca2+ permeability. Founder line B17-2 has high effector and lacZ expression in heart (SA and AV nodes). (J:314125)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
All:	1 reference(s)