Pax6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:266589 Cohen-Tayar Y, et al., Pax6 regulation of Sox9 in the mouse retinal pigmented epithelium controls its timely differentiation and choroid vasculature development. Development. 2018 Aug 9;145(15):dev163691
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6293892
Gene symbol:	Pax6
Gene name:	paired box 6

Antibody:	Anti-Pax6 (AD2.38 sc-32766)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1A ©

Specimen 1A: embryonic day 15.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS23: pigmented retinal epithelium	Present		1A

2A ©

Specimen 2A: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		2A

2B ©

Specimen 2B: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm, Tg(Dct-cre)1Apdn/0 (more

)
Note: Conditional mutant. Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		2B	No difference was noted in Pax6 expression level in the Sox9 cKO.

S1B ©

S1F ©

Specimen S1F: embryonic day 13.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present	Widespread	S1F , S1B	In the early optic cup, Pax6 was detected throughout the retinal pigmented epithelium.

S1C ©

S1G ©

Specimen S1G: embryonic day 15.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS23: pigmented retinal epithelium	Present		S1C , S1G

S1D ©

S1H ©

Specimen S1H: embryonic day 19.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS26: pigmented retinal epithelium	Ambiguous		S1D , S1H

S1E ©

S1I ©

Specimen S1I: postnatal day 8 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Ambiguous		S1E , S1I	Pax6 was gradually replaced by Sox9 in the retinal pigmented epithelium.

S2A ©

S2A' ©

S2B ©

S2B' ©

Specimen S2A/B: embryonic day 13.5; Pax6^tm2Pgr/Pax6^tm2Pgr (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		S2B , S2A' , S2A , S2B'

S2D ©

S2D' ©

S2E ©

S2E' ©

Specimen S2D/E: embryonic day 13.5; Pax6^tm2Pgr/Pax6^tm2Pgr (more

)
Note: Conditional mutant. Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Absent		S2D , S2D' , S2E , S2E'

S4A ©

S4A' ©

S4B ©

S4B' ©

Specimen S4A/B: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		S4B' , S4A , S4B , S4A'

S4D ©

S4D' ©

S4E ©

S4E' ©

Specimen S4D/E: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm, Tg(Dct-cre)1Apdn/0 (more

)
Note: Conditional mutant. Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		S4D' , S4E' , S4E , S4D	No difference was noted in Pax6 expression level in the Sox9 cKO.

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