Probe:	Livin probe2
Probe preparation:	labelled with alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: head	Absent		4A

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Trace		4B	Expression is barely above background.

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Strong	Regionally restricted	4C , 4D	Expression was strong in primary fiber cells located in the center of the lens.
TS24: eye	Strong	Regionally restricted	4C , 4D	Expression was strong in primary fiber cells in the center of the lens; it was undetectable elsewhere in the eye.

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Regionally restricted	4E	Expression was restricted to nucleated inner cortical fiber cells bordering the central organelle-free zone of the lens.
TS28: lens	Present	Regionally restricted	4E	Expression was in fiber cells but was not detected within the anucleated cells of the organelle-free zone.
TS28: lens anterior epithelium	Absent		4E

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Regionally restricted	4F	Expression was restricted to a thin layer of fiber cells adjacent to the organelle-free zone of the lens.
TS28: lens	Present	Regionally restricted	4F	Expression was in fiber cells but was not detected within the anucleated cells of the organelle-free zone.
TS28: lens anterior epithelium	Absent		4F