Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:27778
Obama H, et al., Midkine (MK) expression in extraembryonic tissues, amniotic fluid, and cerebrospinal fluid during mouse embryogenesis. J Biochem. 1995 Jul;118(1):88-93
|
Assay type:
|
Northern blot
|
MGI Accession ID:
|
MGI:6287667
|
Gene symbol:
|
Ptn
|
Gene name:
|
pleiotrophin
|
Probe:
|
Ptn cDNA2
|
Probe preparation:
|
Double stranded labelled with P32 DNA
|
Visualized with:
|
Autoradiography
|
Assay notes:
|
Fig. 2 shows the relative intensity of the signals after normalization as to the signal of Actb.
|
|
Results
|
Image: 1B PTN
E7.5
|
TS28: uterus
|
Present
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
E9.5
|
TS28: uterus
|
Weak
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
E11.5
|
TS28: uterus
|
Trace
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
E13.5
|
TS28: uterus
|
Trace
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
E15.5
|
TS28: uterus
|
Weak
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
E17.5
|
TS28: uterus
|
Weak
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
P
|
TS28: uterus
|
Weak
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
non-pregnant mice
|
E11.5
|
TS28: placenta
|
Trace
(a)
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
E13.5
|
TS28: placenta
|
Trace
(a)
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
E17.5
|
TS28: placenta
|
Ambiguous
(a)
|
20 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Female |
|
Notes:
|