Nras Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:27714 Lopez-Fernandez LA, et al., Different developmental pattern of N-ras and unr gene expression in mouse gametogenic and somatic tissues. Biochim Biophys Acta. 1995 Jul 25;1263(1):10-6
Assay type:	Northern blot
MGI Accession ID:	MGI:6287299
Gene symbol:	Nras
Gene name:	neuroblastoma ras oncogene

Probe:	Nras probe1
Probe preparation:	labelled with P32
Visualized with:	Autoradiography

Results

Image: 3_N-ras

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	5 kb (b)	1.3 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
fetal	E15.0 (a)	TS23: testis	Trace	Trace	15 µg; total RNA	Swiss		Male
0	P0	TS27: testis	Weak	Weak	15 µg; total RNA	Swiss		Male
3	P3	TS27: testis	Present	Present	15 µg; total RNA	Swiss		Male
6	P6	TS28: testis	Present	Present	15 µg; total RNA	Swiss		Male
10	P10	TS28: testis	Present	Present	15 µg; total RNA	Swiss		Male
14	P14	TS28: testis	Present	Present	15 µg; total RNA	Swiss		Male
18	P18	TS28: testis	Present	Present	15 µg; total RNA	Swiss		Male
22	P22	TS28: testis	Present	Present	15 µg; total RNA	Swiss		Male
30	P30	TS28: testis	Present	Present	15 µg; total RNA	Swiss		Male
adult	P adult	TS28: testis	Present	Present	15 µg; total RNA	Swiss		Male

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The 5.0 kb transcript was more abundant than the 1.3 kb transcript. There was not significant variation in the expression level during postnatal development of testis.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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