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Gene Expression Data
Assay Details
Assay
Reference: J:270658 Wang XW, et al., Lin28 Signaling Supports Mammalian PNS and CNS Axon Regeneration. Cell Rep. 2018 Sep 4;24(10):2540-2552.e6
Assay type: RT-PCR
MGI Accession ID: MGI:6285145
Gene symbol: Lin28b
Gene name: lin-28 homolog B
Probe: Lin28b-pG, Lin28b-pH
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative levels of mRNAs were determined using the ddCt method and normalized to control. Gapdh was used as the endogenous control.
Results
Image: S1B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
E15 E15.0 (a) TS23: L4 dorsal root ganglion
TS23: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
E18 E18.0 (a) TS26: L4 dorsal root ganglion
TS26: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
P0 P0 TS27: L4 dorsal root ganglion
TS27: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
P3 P3 TS27: L4 dorsal root ganglion
TS27: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
P7 P7 TS28: L4 dorsal root ganglion
TS28: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
P14 P14 TS28: L4 dorsal root ganglion
TS28: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
P21 P21 TS28: L4 dorsal root ganglion
TS28: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
P28 P28 TS28: L4 dorsal root ganglion
TS28: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
P56 P56 TS28: L4 dorsal root ganglion
TS28: L5 dorsal root ganglion 		Present Not Specified; total RNA Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) RNA levels dropped from E15 to E18 and remained at low level through P56.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory