Probe:	Fgf10 probe 41
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS14: otic placode	Weak	Regionally restricted	1A	Weak expression in anterior region of otic placode just as it begins to invaginate.

Structure	Level	Pattern	Image	Note
TS14: otic pit	Present	Regionally restricted	1B	As otic placode sinks below surface ectoderm to form the otic cup, expression is upregulated but remains restricted to anterior region.

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Present	Regionally restricted	1C	As otic cup begins to approach closure, expression in anterior epithelium but extends more ventrally in otic epithelium.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	1D	Upon closure to form otocyst, expression throughout otic epithelium, appearing stronger in anterior-ventral region

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	1E	Expression remains prominent in anterior ventral otocyst.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Strong	Regionally restricted	1F	Strong anterior-ventral expression domain is predominantly medial, located on the neural tube side.