Afp RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:242505 Jiang J, et al., Zhx2 (zinc fingers and homeoboxes 2) regulates major urinary protein gene expression in the mouse liver. J Biol Chem. 2017 Apr 21;292(16):6765-6774
Assay type:	RT-PCR
MGI Accession ID:	MGI:6284382
Gene symbol:	Afp
Gene name:	alpha fetoprotein

Probe:	Afp-pK1, Afp-pL1
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression is shown compared to levels at E17.5 (which was set as 1) and normalized to L30 expression.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
e17.5	E17.5	TS25: liver	Present	1 µg; total RNA	involves: C3H * C57BL/6		Male
p1	P1	TS27: liver	Present (a)	1 µg; total RNA	involves: C3H * C57BL/6		Male
p7	P7	TS28: liver	Present (b)	1 µg; total RNA	involves: C3H * C57BL/6		Male
p14	P14	TS28: liver	Present (c)	1 µg; total RNA	involves: C3H * C57BL/6		Male
p21	P21	TS28: liver	Present (d)	1 µg; total RNA	involves: C3H * C57BL/6		Male
p28	P28	TS28: liver	Present (e)	1 µg; total RNA	involves: C3H * C57BL/6		Male
p56	P56	TS28: liver	Present (f)	1 µg; total RNA	involves: C3H * C57BL/6		Male

Notes:
(a) Expression was 0.63 fold to that seen at E17.5.
(b) Expression was 0.24 fold to that seen at E17.5.
(c) Expression was 0.002 fold to that seen at E17.5.
(d) Expression was 0.0002 fold to that seen at E17.5.
(e) Expression was 0.00007 fold to that seen at E17.5.
(f) Expression was 0.00003 fold to that seen at E17.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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