Antibody:	anti-NSDHL
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	2B , 2A	Expressed in the basal column, with less signal in the mantle column.
TS17: dorsal root ganglion	Strong		2B , 2A
TS17: liver	Strong	Regionally restricted	2C , 2A	Expressed in a subset of cells.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	2D	High level of staining in the basal column of the neural tube relative to the mantle column and ventricular layer.
TS17: dorsal root ganglion	Strong		2D

Structure	Cell Type	Level	Pattern	Image	Note
TS22: dorsal root ganglion		Strong		2F
TS22: liver		Strong		2F
TS22: brain		Present		2F	Signal is higher than in other tissues such as heart and lung.
TS22: heart		Present		2F
TS22: lung		Present		2F
TS22: Leydig cell	Leydig cell	Strong		2F , 2G
TS22: rib cartilage condensation		Strong	Regionally restricted	2H , 2F	Expressed in the condensing mesenchyme surrounding the chondrocytes, specifically in the dorsal/caudal region of the embryo.
TS22: neocortex		Present		2I , 2F	Expressed as a continuous band of stained neurons throughout the cortex, including the dendrites of neurons.
TS22: superior cervical ganglion		Very strong		2F
TS22: trigeminal ganglion		Very strong		2F

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Patchy	2J	Patchy staining pattern, with radiating sectors of neurons showing no staining.

Structure	Level	Pattern	Image	Note
TS23: retina layer	Strong	Regionally restricted	2K	Expressed in the ganglion cell layer, with lower signal throughout the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS23: retina layer	Present	Patchy	2L	Mosaic expression in the ganglion cell layer with radiating sectors of positive and negative cells in progeny cells.

Structure	Level	Pattern	Image	Note
TS28: liver	Strong	Widespread	3A	Expressed throughout the lobule, with slightly higher signal in the pericentral region.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Patchy	3B , 3C	Mosaic pattern of staining in hepatocytes. There were small clusters of hepatocytes that did not stain.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Patchy	3D	Mosaic pattern of staining in hepatocytes. A relatively large number of negative hepatocytes were seen.

Structure	Level	Pattern	Image	Note
TS28: liver	Present		3E	Mosaic pattern of staining in hepatocytes, with random distribution of positive and negative cells.

Structure	Level	Pattern	Image	Note
TS28: liver	Present		3F	Mosaic pattern of staining in hepatocytes, with random distribution of positive and negative cells.

Structure	Level	Pattern	Image	Note
TS27: sebaceous gland	Strong		4A
TS27: epidermis	Weak		4A
TS27: hair inner root sheath	Moderate		4A
TS27: hair root sheath matrix	Moderate		4A
TS27: hair outer root sheath	Present	Regionally restricted	4A	No staining at the level between the arrector pili muscle and the sebaceous gland, the bulge.

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present	Patchy	4B	Mosaic staining pattern, with regions lacking staining.
TS27: hair follicle	Present	Patchy	4B	Mosaic staining pattern, with regions lacking staining.

Structure	Level	Pattern	Image	Note
TS28: hair inner root sheath	Very strong		4C , 4E
TS28: hair outer root sheath	Very strong		4C , 4E
TS28: sebaceous gland	Very strong		4C , 4E

Structure	Level	Pattern	Image	Note
TS28: epidermis	Present	Patchy	4F , 4D	Mosaic staining pattern, with regions lacking staining.
TS28: hair follicle	Present	Patchy	4F , 4D	Mosaic staining pattern, with regions lacking staining.

Structure	Level	Pattern	Image	Note
TS28: hair inner root sheath	Strong	Patchy	4G	Mosaic staining pattern, with regions lacking staining.
TS28: hair outer root sheath	Strong	Patchy	4G	Mosaic staining pattern, with regions lacking staining.
TS28: hair root sheath matrix	Strong	Patchy	4G	Mosaic staining pattern, with regions lacking staining.
TS28: sebaceous gland	Strong	Patchy	4G	Mosaic staining pattern, with regions lacking staining.
TS28: epidermis	Weak	Patchy	4G	Mosaic staining pattern, with regions lacking staining.

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Present	Regionally restricted	5A , 5C	Expressed in a wide band of differentiating neurons in the outer layer. The cell bodies as well as dendrites were stained.
TS27: hippocampus	Present	Widespread	5A , 5E	Expressed in virtually all of hippocampal neurons.
TS27: thalamus	Present	Widespread	5E , 5A
TS27: main olfactory bulb mitral cell layer	Present		5A
TS27: caudate-putamen	Present		5A	relatively low staining
TS27: cerebral cortex intermediate zone	Present		5A	relatively low staining
TS27: cerebral cortex subventricular zone	Present		5A	relatively low staining
TS27: choroid plexus	Present		5A	relatively low staining
TS27: dentate gyrus	Present		5A	relatively low staining
TS27: main olfactory bulb granule cell layer	Present		5A	relatively low staining
TS27: superior colliculus	Present		5A	relatively low staining

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Present	Patchy	5B , 5D	Mosaic staining pattern, with radial sectors of positive and negative neurons.
TS27: hippocampus	Present	Patchy	5F , 5B	Mosaic staining pattern, with a mixture of stained and unstained cells throughout its length.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5G	Highest expression in a layer of relatively large pyramidal neurons. The proportion of strongly positive cells was lower in the adult. Mainly in the cell bodies of neurons, with much fainter staining in the dendrites.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5H	Indistinguishable from the wild-type brain with regard to the pattern of staining.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		5I
TS28: hippocampus CA3	Present		5I

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		5J	Indistinguishable from the wild-type brain with regard to the pattern of staining.
TS28: hippocampus CA3	Present		5J	Indistinguishable from the wild-type brain with regard to the pattern of staining.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Uniform	S1A	Relatively low expression.
TS9: extraembryonic ectoderm	Present	Uniform	S1A	Relatively low expression.
TS9: ectoplacental cone	Trace		S1A	Little or no staining.
TS9: trophoblast giant cell	Trace		S1A	Little or no staining.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		S1A

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Uniform	S1B	Roughly equivalent staining throughout the structures.
TS11: extraembryonic component	Present	Uniform	S1B	Roughly equivalent staining throughout the structures.
TS11: trophoblast giant cell	Present		S1B	Weakest expression.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Strong		S2
TS22: dorsal root ganglion	Strong		S2
TS22: liver	Strong		S2
TS22: intestine epithelium	Moderate		S2
TS22: maturing glomerular tuft	Moderate		S2
TS22: renal tubule	Moderate		S2

Structure	Level	Pattern	Image	Note
TS24: epidermis	Weak	Regionally restricted	S4A	Expressed in the outermost layer.
TS24: hair follicle	Trace		S4A	Very faint signal in germ stage hair follicles.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Patchy	S4B	Mosaic staining pattern, with regions lacking staining.
TS24: hair follicle	Present	Patchy	S4B	Mosaic staining pattern, with regions lacking staining.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Moderate	Widespread	S5C , S5A	Expressed in virtually all of the neurons.
TS28: hippocampus CA1	Moderate		S5C , S5A	Expressed in virtually all of the neurons.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Non-Uniform	S5B , S5D	Mosaic staining pattern, with a mixture of stained and unstained cells.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Moderate	Widespread	S5E	Evenly stained throughout its length.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present	Non-Uniform	S5F	Mosaic staining pattern, with a mixture of stained and unstained cells.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present			Distinctly higher expression than those in other tissues.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present			Staining was strongest in the layer of cells composed of Purkinjie cell precursors and Bergmann glia.