Probe:	mSim1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		8A

Structure	Level	Pattern	Image	Note
TS12: unsegmented mesenchyme	Present		8B

Structure	Level	Pattern	Image	Note
TS13: unsegmented mesenchyme	Present		8C

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present	Uniform	8D

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	8E	Expressed in the ventrolateral regions of the rostral mesencephalon.
TS15: hindgut	Present		8E
TS15: neural tube lateral wall	Present	Regionally restricted	8E	Expressed in the ventrolateral region of the caudal neural tube.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	8F	Expressed in the ventrolateral region but not in the ventral midline of the mesencephalon.

Structure	Level	Pattern	Image	Note
TS15: dermatome	Present		8G
TS15: nephrogenic cord	Present		8G
TS15: myotome	Absent		8G
TS15: sclerotome	Absent		8G
TS15: neural tube lateral wall	Present	Regionally restricted	8G	Expressed in the ventrolateral region of the caudal neural tube.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8H	Expressed in the zona limitans intrathalamica.
TS17: forebrain-midbrain boundary region	Present	Regionally restricted	8H	Expressed in the ventrolateral domain of the pretectum.
TS17: dermatome	Present		8H
TS17: hindgut	Present		8H
TS17: midbrain	Present		8H
TS17: nephrogenic cord	Present		8H
TS17: myotome	Absent		8H
TS17: sclerotome	Absent		8H
TS17: neural tube lateral wall	Present	Regionally restricted	8H	Expressed in the ventrolateral region of the caudal neural tube.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Strong	Regionally restricted	8I	Expressed in the neuroephithelium of the ventrolateral portion of the mesencephalon.
TS17: diencephalon	Present	Regionally restricted	8I	Expressed in the zona limitans intrathalamica.
TS17: forebrain-midbrain boundary region	Present	Regionally restricted	8I	Expressed in the ventrolateral domain of the pretectum.

Structure	Level	Pattern	Image	Note
TS17: dermatome	Present		8J
TS17: neural tube lateral wall	Present	Regionally restricted	8J	Expressed in the ventrolateral domain of caudal neural tube between the floor plate and motor neurons.
TS17: myotome	Absent		8J
TS17: sclerotome	Absent		8J

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	9A	Expressed in the zona limitans intrathalamica, caudal diencephalon and the ventral thalamus. Within the regions in which Shh was expressed.
TS17: forebrain-midbrain boundary region	Present	Regionally restricted	9A	Expressed in the ventrolateral domain of the pretectum.
TS17: midbrain	Present	Regionally restricted	9A	Expressed in the ventrolateral side of the rostral mesencephalon. Within the regions in which Shh was expressed.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	9D	Expressed in the zona limitans intrathalamica. Within the regions in which Shh was expressed.